Tumor Vascular Markers： Possibilities for Detection of Breast Carcinoma.pdf

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Tumor Vascular Markers： Possibilities for Detection of Breast Carcinoma.pdf

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J．Chem．Chem．Eng．6(2012)118-123 TumorVascularMarkers：POSS_bilitieSforDetectionof BreastCarcinoma MiroslavaBilecov~i．Rabajdov~i，PeterUrban ，AndreaGre~ovfi2，AnnaBirkov~i，AlexanderOstr6andM~tria Marekov~i1 1．DepartmentofChem~try,Biochemistry,MedicalBiochemistryandLABMED,FacultyofMedicine．P．J．~afdrikUniversity, Kogice04154,Slovakia 22ndDepartmentsofGynecologyandObstetrics,FaculytofMedicine,PjSafdrikUniversiyt,Kogice04154,Slovakia Received：October11，2011／Accepted：November07，2011／Published：February25，2012 Abstract：Breastcancerisoneoftheworld’smosturgenthealthproblems．Thefirstsymptomsofmammarymalignanciesare manifestedonlyatna advnacedstagewithsignificantmo~ality．Detectingthisdiseaseatanearlystagegivesthemajorityofpatientsa beaerchanceofsurviva1．Theaimwastosearchforchnagesingeneexpressionofspecifictumorvascularmrakerslikedeathreceptor 6(Dr6)andglycoproteinM6B(Gpm6B)inthebloodofpatientswithbreastcancer．Allsubjectsweredividedintotwogroups．First groupwithpatientsracwithdifferentgradesofbreatstumors(n=30)．Secondgroupconsistsfromhealthywomen(n=15)．After isolationofmRNA rfom blood．RT—PCRwasfollowedbygelelectrophoresis．Forstatisticalanalysisone．wayANOVA was usedwith Student’sTtestusingGraphPadInStatsoftwrae．SignificnatchangesinmRNA levelsofgeneDr6inallgradesoffirststagebreast cnacerweredetected．ThemI A levelsofDr6showedarisingtendencyrfomGlfll6％highervaluethancontro1)toG3(198％ higherthancontro1)．DuringmonitoringofhtemRNAlevelofGpm6B．aweakerincreasewasobservedthaninDr6．Thedifferencein Glwasonly8％ highercompraedwithcontrolsand44％ atG3．From ourresultsitcallbeconcludedthatDR6isamoresuiatble markerforthediagnosisofbreastmalignnaciesintheeralystagesthna Gpm6B．Inourwork．anon-invasivemethodformoretimely na dprecisedeterminationofhteeralierstagesofbreastcancerisdescribed．whichcouldalsocontributetomonitoringtheeffectiveness ofrteatment，orregressionofthisdisease． Keywords：RT-PCR，Dr6，Gpm6B，gynecologicalmalignancies，breastcnacer 1．IntrOducti0n