糖脂消对胰岛素抵抗高血压大鼠基因表达谱的影响.docVIP

精品论文 参考文献 糖脂消对胰岛素抵抗高血压大鼠基因表达谱的影响 The Influence of Tangzhixiao on Insulin Resisting Hypertension Rats Gene Expression Profile王波淤WANG Bo曰李玉明于LI Yu-ming曰赵旭燕于ZHAO Xu-yan曰蔡绍皙盂CAI Shao-xi曰张延威榆ZHANG Yan-wei（淤贵州师范学院实验教学管理中心，贵阳550018；于武警后勤学院平津医院心血管病研究所，天津300162；盂重庆大学生物工程学院生物力学与组织工程教育部重点实验室，重庆400044；榆贵州师范学院贵州省生物资源开发利用特色重点实验室，贵阳550018）（淤Experiment Teaching Management Center，Guizhou Normal College，Guiyang 550018，China；于Cardiovascular Institute，Pingjin Hospital，Logistics University of Peoples Armed Police Force，Tianjin 300162，China；盂Key Laboratory of Ministry of Education of Biomechanics amp; Tissue Engineering，Bioengineering College of Chongqing University，Chongqing 400044，China；榆Guizhou Key Laboratory of Biological Resources Development and Utilization characteristics，Guizhou NormalCollege，Guiyang 550018，China） 　　摘要 院目的：利用基因芯片技术探讨糖脂消对胰岛素抵抗高血压大鼠基因表达谱的变化，研究糖脂消治疗胰岛素抵抗高血压的作用机制。方法：用高果糖饲料诱发SD 大鼠胰岛素抵抗模型，给予糖脂消口服后，用基因芯片分别检测高果糖组、治疗组，计算机软件分析后，观察基因表达的变化。结果：治疗组表达差异的基因有95 条，新基因有23 条，已知基因34 条。结论：糖脂消可以改变其基因表达谱，为进一步探讨糖脂消治疗作用创造了条件。 　　Abstract: Objective: The gene chip technology is used to study the changes of Tangzhixiao on insulin resisting hypertensive rats geneexpression profile, and the mechanism of the effect of Tangzhixiao on Insulin Resisting Hypertension is studied. Methods: The high fructosediet is used to induce SD rats insulin resistance model, and after giving the rats Tangzhixiao, the high fructose group and the treatmentgroup are tested by the gene chip and the changes of gene expression is observed through the computer software analysis. Results: There are95 genes with differential expression, 23 new genes and 34 known genes in the treatment group. Conclusion: Tangzhixiao can change thegene expression profile, creating conditions for further exploring the treatment effect of Tangzhixiao. 　　关键词院糖脂消；基因芯片；胰岛素抵抗；基因表达谱Key words: Tangzhixiao；gene chip；insulin resistance；gene expression profile中图分类号院R587 文献标识码院A 文章编号院1006-4311（2014）25-0327-02 　　0 引言本文在查阅国内外有关文献的基础上，利用基因芯片技术，进行了糖脂消治疗胰岛