读书报告2017.pptVIP

* * * * * * EEPD1 Is a Novel LXR Target Gene in Macrophages WhichRegulates ABCA1 Abundance and Cholesterol Efflux Reporter : Chong Huang (黄冲) Supervisor : Chao-ke Tang (唐朝克 教授) Mar. 7, 2017 Institute of Cardiovascular disease, Key Laboratory for Arteriosclerology of Hunan Province, University of South China Jessica Kristine Nelson, Duco Steven Koenis, Saskia Scheij, Emma Clare Laura Cook, Martina Moeton, Ana Santos, Jean-Marc Lobaccaro, Silvere Baron, Noam Zelcer Arterioscler Thromb Vasc Biol IF：5.969 Jan. 2017 Background Background 3 ABCA1 LXR macrophage ? Objective To identificy the genes that contribute to LXR-stimulated cholesterol efflux. 4 Results * Transcriptional profiling of liver X receptors (LXR) activation in human THP1 macrophages. EEPD1 is a direct liver X receptors (LXR) target gene in human and mouse macrophages. A liver X receptors–responsive element (LXRE) in intron 2 of EEPD1 drives LXR-dependent expression. EEPD1 is anchored to the plasma membrane. EEPD1 silencing decreases Apo A1-dependent cholesterol efflux and ABCA1 abundance. Result 1 * Transcriptional profiling of liver X receptors (LXR) activation in human THP1 macrophages Result 2 * EEPD1 is a direct liver X receptors (LXR) target gene in human and mouse macrophages Result 2 10 Figure 2. Transcriptional profiling identifies liver X receptors (LXR)–regulated genes in THP1 macrophages. A ABCA1, ABCG1, LXRα , SMPDL3A, and IDOL Established LXRresponsive Genes： EEPD1, PPF1A2, IQSEC1, and PBX4 Additional genes: SMPDL3A:鞘磷脂酸磷酸二酯酶3 IDOL:低密度脂蛋白诱导性降解器 EEPD1：核酸内切酶 - 核酸外切酶 - 磷酸酶家族结构域1 Result 2 * B Result 2 12 Figure 3. Endonuclease–exonuclease–phosphatase family domain containing 1 (EEPD1) is a