转化生长因子β1和胰岛素对人鼻中隔软骨细胞增殖和分化影响的研究.pdfVIP

耳鼻咽喉一头颈外科2003年H月第10卷第4期 ChineseArchOtolaryngolHeadNeckSurgAugust2003,Vol10,No.4 -235- 终扒片价耳协一护-一丫一下铆. 代味洲、一、东渺宏脚缺脉基础研究 转化生长因子-01和胰岛素对人鼻中隔软骨细胞增殖和分化影响的研究 李景红’，黄金中’，杜 江’ (第 一军医大学珠江医院,1.耳鼻喉科，2.全军儿科中心实验室，广东广州 510282) 夕 抓讯住嚼俩针忿汽姚认讯功么如忿户叭万呱赫骗份 蘸狄氢隘纸鬓焦髦 摘〔 要〕 目的 了解转化生长因f-P1(transforming [ABSTRACT] Objective Tostudytheeffectsof growthfactor-betaI,TGF-PI)和胰岛素对人鼻中隔软骨细胞 transforminggrowthfactor01andinsulinonthepro- 增殖和分化的影响·方法体外培养人鼻中隔软骨细胞，采用四 liferationanddiffertiationofhumannasoseptalchondro- 甲基偶氮陛蓝(MTT)代谢和S一Na,SO,掺入的检测比较不 cytesculturedinvitro.MethodsTheproliferativeand 141浓度TGF-Pt和胰岛素对人奥中VM软骨细胞的增殖以及软 proteoglycansynthesisefficienciesofTGF-P1andin- 骨基质蛋白多糖合成的影响，观察TGF-P1和胰岛素对软骨细 sulinonhumannasoseptalchondrocytesculturedinvit- 胞表1l1的影响。结果在15%血清的培养条件下，TGF-P、和 roweremeasuredbyusingMTT intakingand 胰岛素均能显著促进软骨细胞增殖，且在各自一定的浓度范围 S-Na2SO,incorporation;phenopyteofhumannasosep- 内呈量效关系，联合作用效果累加:TGF-PI,TGF-P1和胰岛 talchondrocyteswereobservedbythejoinedofTGF- 素联合作用促使软骨基质蛋自多糖的合成量明显提高; P1andinsulin.ResultsIn15%serumcontaining TGF-P1使传代软骨细胞去分化提前·结论一定浓度的 medium,TGF-P1andinsulincouldenhancetheprolif- TGF-P1,胰岛素和TGF-P1+胰岛素对体外培养的人鼻中隔 erationofhumannasoseptalchondrocytessignificantly 软骨细胞具有显著的促增殖作用。 andhadmitogeniceffectandacteddose-dependently. 关〔键词」鼻中隔:软骨:胰岛素;转化生长因子 Thesynergeticeffectswereachievedwhentheyareused 周 ‘户 incombination.TGF一。1andcombiningeffectsof Effectsoftransforminggrowthfactor01and TGF-01andinsulincouldimprovetheproteoglycan insulinontheproliferationanddifferentiationof synthesis. TGF一01acceleredthedediffertiationof humannasoseptalchondrocytesculturedinvitro chondrocytes.ConclusinTGF-01,insulinandcombi- LlJingho耐，HUANGJinzho4,DUAanj nationofbothatappropriateconcentrationscansignifi- (1DeparhnentofOtolaryngology,2PediatricsCenterLaboratory cantlyenhencetheproliferationofhumannasoseptal ofPLA,AffiliatdZhujiangHospitalofFirst