蒲公英绿原酸β-环糊精包合物的鉴定及抗氧化作用的研究-食品科学.pdfVIP

30 2007, Vol. 28, No. 03 食品科学 ※基础研究 沈 奇，吴晓慧，王 敏，沈小青，任 勇，吴国荣* (南京师范大学生命科学学院， 江苏 南京 210097) ：本文在蒲公英绿原酸分离纯化的基础上，对其进行了β- 环糊精的包合和鉴定，比较研究绿原酸及其环 糊精包合物(β-CD -CA)的抗氧化作用：测定它们在化学模拟条件下抑制超氧阴离子(O－ 2 ·)、羟自由基(·OH)形成 及抑制紫外线(UV)诱导红细胞溶血的效应和对猪油在高温贮藏下过氧化值(POV)与酸价(AV)升高的影响。结果表明， 绿原酸及其包合物都能够有效清除O－ 2 ·、·OH、且能有效降低UV 诱导的红细胞(RBC)的溶血，显著延缓猪油 POV 以及AV 值的增加。相比较β-CA 的抗氧化作用较CA 更好，并且在一定程度超过阳性对照物茶多酚的作用。 环糊精包合物比前者有更有效的抗氧化作用的原因可能与包合后对光和氧稳定相关。 ：蒲公英；绿原酸；包合；β- 环糊精；抗氧化 Study on Identification and Anti-lipid Peroxidation Effects of β-cyclodextrin Inclusion of Chlorogenic Acid Extracted from Dandelion SHEN Qi，WU Xiao-hui，WANG Min，SHEN Xiao-qing，REN Yong，WU Guo-rong* (College of Life Science, Nanjing Normal University, Nanjing 210097, China) Abstract ：On the basis of separation and purification of chlorogenic acid(CA) in dandelion，the β-cyclodextrin(β-CD) inclusion of CA and identification were studied in this paper. The comparison of the differences of the anti-lipid peroxide effects between CA and its inclusion(β-CD -CA) was carried out. The conditions of the chemical simulation, the clearing function on － the form of superoxide anion(O2·) and hydroxyl radical(·OH), the inhibition on UV-induced hemolysis of red blood cell(RBC) and the increase of peroxide value(POV) and acid value(AV) of oil in the storge at high temperature of CA andβ-CA were evaluated － in this paper too. The results showed that both CA and β-CA have clearing function on O2· and ·OH, and effective inhi