原创力文档DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1英文文献.pdfVIP
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letters to nature
Cell adhesion and migration 27. Gray, A., Olsson, H., Batty, I. H., Priganica, L. Peter Downes, C. Nonradioactive methods for the
For adhesion assays, cells starved of cytokines for 24 h were washed and re-suspended in assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of
4 per well in a 50-ml volume signaling lipids in cell and tissue extracts. Anal. Biochem. 313, 234–245 (2003).
culture medium without cytokines. Cells were plated at 5 £ 10
in 96-well plates pre-coated overnight with 5 mg ml21 human plasma fibronectin (Gibco). 28. Okayama, Y., Tkaczyk, C., Metcalfe, D. D. Gilfillan, A. M. Comparison of Fc1RI- and Fcg RI-mediated
After 30 min of stimulation with or without SCF (in 100 ml final volume), non-adherent degranulation and TNF-a synthesis in human mast cells: selective utilization of phosphatidylinositol-3-
cells were removed by inverting plates for 15 min. The adherent cells were lysed using kinase for Fcg RI-induced degranulation. Eur. J. Immunol. 33, 1450–1459 (2003).
HTAB and b -hexosaminidase activity measured to assess the fraction of adherent cells. 29. Tilley, S. L., Wagoner, V. A., Salvatore, C. A., Jacobson, M. A. Koller, B. H. Adenosine and inosine
Adhesion is expressed as percentage of input. For migration assays, exponentially growing increase cutaneous vasopermeability by activating A(3) receptors on mast cells. J. Clin. Invest. 105,
cells were washed and re-suspended in Tyrode
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