纤细席藻对重金属镍胁迫的响应研究.docVIP

纤细席藻对重金属镍胁迫的响应研究 陈兰洲汪静武艳芳柯檀梁亚楠刘思瑶 武汉大学资源与环境科学学院湖北省环境修复技术 研究中心武汉文科生态环境有限公司 实验研宄丫纤细席藻Phormidium tcnuc对重念属線胁迫的光合生理响应及外源 抗氧化剂的保护作用，结果发现:在不同浓度Ni2+短期处理纤细席藻时，其荧光 产量随处理时间先发生急剧下降，随后有所回升;荧光产量随着Ni2+浓度的增加 而明显降低，在100 mg/L Ni21浓度下荧光曲线的0、J、I、P相完全消失，光合 活性（Fv/Fm）丧失.经抗氧化剂抗坏血酸（ASC）和N-乙酰半胱氨酸（NAC）分 别预处理的纤细席藻荧光产量有一定程度恢复，且ASC抗氧化作用相比NAC更加 明显，但其保护作用均随着时间增加而逐渐减弱.在不同浓度的Ni2+长期胁迫时， Id内抑制作用显著，叶绿素a、光合活性及电子传递链光合参数（ET0/ABS、 ETO/TRO、RC/CS0）下降显著，SOD活性升高.实验结果表明:镍对纤细席藻的光 合生理活性具有较强的抑制作用，而外源抗氧化剂可以短期内有效缓解重金属 镍胁迫造成的损伤，因此在吸附去除重金属时可有效提高藻类的吸附效率和循 环利用. 关键词： 纤细席藻;镍;叶绿素荧光动态;抗氧化系统; 陈兰洲（1976-）,男，教授，生导师，，研究方向:环境生 物学，E-mail:chcnlz@whu. edu.cn 2017-09-12 基金：国家自然科学基金资助项目 The Responses of Phormidium tenue on the Stress of Heavy Metal Nickel Chen Lanzhou Wang Jing Wu Yanfang Ke Tan Liang Yanan Liu Siyao School ofRe source Environniental Science, Hubei Research Center of Environment Remediation Technology， Wuhan University; Wuhan Wenke Ecological Environment Ltd.; Abstract： The effects of nickel on Phormidium tenue, including photosynthetic activity, antioxidant enzymes and carbohydrate metabolism were investigated.It was found that fluorescence intensity decreased rapidly in the first 10 min after Ni2. was added into the system, and partly recovered to the normal. \i contents could inhibit the fluorescence production of P- tenue and aggravated as the increase of Ni concentration, even O-I-J-P phases of chlorophyll a fluorescence transients disappeared absolutely as well as Fv/Fni with 100 mg/L Ni21 . The two antioxidants, ASC and NAC，were able to relieve the stress of Ni2 on P. tenue, and ASC was better at protecting capacity，while the anti-oxidation effects decreased as extension of treatment time. The chlorophyll a content, Fv/Fmand photosynthetic parameters (ET0/ABS、ET0/TK0、RC/CS0) decreased significantly after Ni2. stressing for 24 h， meanwhile SOD activity oppositely increased to defend against stress of Ni2. . The results indicated that Ni2+ had an inhibitory effect on photosynthesis of P. tenue, and an