碱性盐胁迫对葡萄杂交砧木荧光特性、叶黄素循环与抗氰呼吸的影响.doc

碱性盐胁迫对葡萄杂交砧木荧光特性、 叶黄素循环与抗氰呼吸的影响 牛彦杰臟ttaM 山东农业大学园艺科学与工程学院/作物生物学国家 重点实验室 以葡萄‘左山一，(Vitis amurensis)与 ‘S04’ (V. berlandieri X V. riparia) 杂交后代中耐碱性盐强的A15、A17与引进砧木中耐碱性盐能力较强的‘1103P’ (V. rupestrisXV. berlandieri)为试材，探讨耐碱性盐能力较强的葡萄砧木的 荧光特性及光保护机制。将组培苗炼苗7周后每天浇灌100 mmol - NaIIC03 (pH 8.62)，测定叶片电导率、丙二醛含量、叶绿素荧光、叶黄素循环及抗氰呼吸速 率。NaHCO3处理8 d后，各株系的叶片电导率与丙二醛(MDA)含量均有大幅升 高;A15、A17的最大光化学效率(FV/FJ、光化学淬灭系数(qp)的降低幅度均 小于‘1103P’，且调节性能量耗散(U上升幅度较大，说明NaHCO3胁迫下 A15、A17的电子传递活性与光抑制程度受影响较小。在NallC03处理K A15、A17 和‘1103P’的叶黄素循环(A+Z) / (A+Z+V)分别比对照提高46. 3%、37.0%、 28. 0%, A15、A17的抗氰呼吸速率在NaHCO3处理下分别比对照提高141. 5%.95. 8%, ‘1103P’则比对照提高63.0%。A15、A17通过依赖叶黄素循环的热耗散与增加 抗氰呼吸速率来减轻光抑制以提高其耐碱性盐能力。 关键词： 葡萄;NaHCO3胁迫;光系统活性;叶黄素循环;抗氰呼吸; 杜远鹏 E-mail: duyua叩eng001@163. com 2017-05-22 基金：现代农业产业技术体系建设专项(CARS-29-zp-2) Effect of alkaline salt stress on fluorescence characteristics, xanthophyll cycle and cyanide-resistant respiration of grape hybrid rootstocks GUO Shu-Hua NIU Yan-Jie ZHAI Heng HAN Ning DU Yuan—Peng College of Horticulture Science and Engineering， Shandong Agricultural University/State Key Laboratory of Crop Biology; Abstract： A15 and A17 [ ‘Zuo Shanl’ (Vitis amurensis) X ‘S04’ (V. berlandieri X V. riparia) ] with strong alkaline salt resistance and rootstock 1103 P’ (V. rupestris X V. berlandieri) with strong alkali resistance in introduced rootstocks were used as materials to study the fluorescence characteristics and photoprotective mechanism under alkaline salt stress. The tissue culture seedlings were irrigated with 100 mmol ? L 1 NaHC03 (pH 8.62) every morning after 7 weeks of acclimatization. Leaf relative electrical conductivity, MDA content, chlorophyll fluorescence, xanthophyl1 cycle, cyanide-resistant respiration rate were determined. After 8 days of NalIC03 treatment, leaf relative electrical conductivity and MDA content of each strain increased significantly. The decrease of Fv/Fk and qP in A15 and A17 were less than that in 1103 I)’，and the increase of Ypoin A15 and A17 were more than that in 1103 P’，in