一株蝗虫病原菌的分离鉴定及其毒力研究有关.doc

一株蝗虫病原菌的分离鉴定及其毒力研究 【摘要】：从蝗虫自然病死虫尸中分离到一株病原菌HR-3，其纯培养物的致病性被科赫原理证明；从内地黄脊竹蝗自然病死的虫尸内分离到一种病原菌，其纯培养物经KOCK病症律证明，并按《伯杰细菌鉴定手册》（第九版）的方法进行了生物学鉴定、生理生化实验，并测定了其DNA中G+C mol含量为63.73%，确定该病原物为类产碱假单胞菌（Pseudomonas pseudoalcaligenes）。为确定该菌在分类学上的地位，经生理生化测定和分子鉴定，此病原菌为粘质沙雷氏菌（Seerratia marcescens）。通过口服感染测定了该菌对蝗虫的毒力，毒力回归方程为y=1.067+0.809x，致死中浓度LC50=1.164×108cfu/ml。为探讨HR-3对蝗虫致病的作用机理，对HR-3感染蝗虫后的组织病理进行了研究，结果表明感染初期蝗虫的中肠上皮细胞受到破坏，继而出现局部变性坏死。感染48h后，消化细胞层大部分区域被空泡状物充盈。 【关键词】：类产碱假单胞菌，粘质沙雷氏菌，鉴定，毒力，病理，蝗虫 【Abstract】：from the locusts natural death worm resin separation to the strains of pathogenic bacteria in HR - 3, the pure cultures of pathogenic by Koch principle; From the mainland huang ridge bamboo locust nature and death of the insect in the resin separation to a pathogen, KOCK disease law has proved that the pure cultures, and press \berger bacteria identification manual\ (ninth edition) method to carry on the biology identification, physiological and biochemical experiments, and the determination of the DNA G + Cmol content is 63.73%, determine the disease original object for the class to produce alkali Pseudomonas (Pseudomonas pseudoalcaligenes). physiological and biochemical and molecular identification of the pathogen of serratia marcescens (Seerratia marcescens). With oral infection to determine the bacterial virulence of locusts, virulence regression equation for y = 1.067 + 0.809 x, lethal concentration LC50 = 1.164 x 108 cfu/ml. To study the role of HR - 3 of locusts and pathogenic mechanism of the HR - 3 infection after locusts histopathologic are studied, the results show that early infection of locusts in intestinal epithelial cells were damaged, and then a local degeneration necrosis. Infection after 48 h, digestive cells most floors are filled with air bubbles. 【Keywords】：pseudomonas class production base, serratia marcescens, appraisal, virulence, pathology, locusts—52. ［4］赵文臣.防治蝗虫问题的反思.科技交流，2000，10：32—34. [5]赵文臣，防治蝗虫问题的反思[J].科技交流，2000.10:32—34. [6]刘世贵，袁兴，朱文，等. 一株蝗虫病原菌的分离和鉴定 [J].微生物学报