(精选)microRNA的作用与调控机制教学课件.pptVIP

SEED ????? ???? ??? ??? ????? ????? ??????? ???? ?????? ??????? ????? ???? ?? SEED ????? 13-130 ?????? ???????. ?????? ?? ??????? ?? Saetrum et. Al. * Neighboring seeds density CLDN12 AAGAAAACTTCTTGTAGCCTCACATTCCCCTTGTGCAAAGAGCTC TAGLN2 TAGATATATATTTTAGCAGTGACATTCCCAGAGAGCCCCAGAGCT ARCN1 GCCACAGTCTGTAATCCATTCACATTCCTCAGTTTCACCACCTCC TIP120A TTTCATTCCGTTTGGATGTCTACATTCCTTATCAAAGGATATAAA PGM2 TTATGTGTTTTACAAAGACCTACATTCCTCATTGTTTCATGTTTG -20 -10 0 10 20 * hsa-miR-302b hsa-miR-507 0.2 0.2 0 0.1 0.1 Seed density of window Seeds density = mean of targets density 0.12 Neighboring seeds density * Corrected α=0.00125 Overlapping seeds (miR binding sites) may interfere with miR function Neighboring seeds contribute to functionality Possibly favored periodical distances between seeds ? miR-mRNA binding pattern * We need a way to determine what is the preferable secondary structure of the miR-target complex RNAcofold from the Vienna package target 5 UUGGCUCACUUGCCUUAGTCATCGCTA 3 miRNA 3 ACCGUAAGUGGCGCACGGAAUU 5 target 5 UUGGUCAUUUUUUUUAUAUUGCCUUAU 3 miRNA 3 ACCGUAAGUGGCGCACGGAAUU 5 target 5 AUCAUAUUAAUUGUGCCUUAUAGTAAT 3 miRNA 3 ACCGUAAGUGGCGCACGGAAUU 5 (a) Using Vienna package RNAcofold we estimated the interaction between the miR and putative targets target 5 U G 3 UGGC UCACU UGCCUUA ACCG AGUGG ACGGAAU miRNA 3‘ UA CGC U 5‘ target 5 U U UUUUUAUAU U 3‘ UGG CAUUU UGCCUUA ACC GUAAG ACGGAAU miRNA 3‘ UGGCGC U 5‘ target 5 A AUAUUAAU U 3‘ UCA UGUGCCUUA AGU GCACGGAAU miRNA 3 ACCGUA GGC U 5 miR-mRNA binding pattern * Bound Not bound target 5 U G 3 UGGC UCACU UGCCUUA ACCG AGUGG ACGGAA