大鼠KCTD10基因的克隆及功能研究.pdfVIP

摘 要 胞生理过程中起重要作用，比如细胞凋产、细胞增殖、B细胞激活、 肝细胞的再生以及…些急性的细胞炎痘反应等。人类的 PDIPI(oolymerasedelta—interactingproteini)是——j}中受TNF—a Channel 全长，命名为KCTDIO(Potassium Domain—containing 被TNF-a诱导的结论， TNFAIPI之间的同源性。Northern IJI 主要在肺里面表达，其次是心脏和睾丸。我们的研究为进一步研究 PDIPI家族的功能提供了基础。 关键词：大鼠，KCTDIO，基因克隆，免疫共沉淀，肿瘤坏死因子a Abstract necrosisfactor a Tumor a(TNF—Q)is that rolesina ofcellular cytokineplays variety processes suchas cell apoptosis，pr01iferation，B andsome regenerationinflammatoryresponses．Human asortof induced 1(PDIPI)is delta—interactingprotein protein TNF—Qandinterleukin caninteract by 6(IL一6)．Besides，PDIPI with cellnuclear the directlypr01iferating of PDIPl isalso smalisubunitDNA poiymerase6(p50)．Thegene foundinrat andhas beenc genome already loned．By theGeneBankESTdatabasetheratPDIPI found using cDNA，we a ofEST thatshared group sequences significant withrat weclonedthefull cDNAofthe PDIPI，and length of it KCTDIO ratPDIPIRACEPCR，namedasrat homologuegene by Thededuced ratPDIPland of