EXPERIMENT-2 DEMONSTRATE THE GENESIS OF 实验2表明，成因.pptVIP

? Li Jingxin Physiologcal Department IMPORTANT QUESTIONS 1.what’s the action potential? 2.how to record it? 1.Intracellular recording 2.Extracellular recording How to prepare a sciatic nerve preparation. . Animal PROCEDURE 1. PITHING Pithing is done to destroy the brain and spinal cord by inserting the pithing needle 2.DISSECTION  1.remove the head and viscera from the body, 2.remove the skin from legs 3.with a pair of scissors the vertebral column is divided into two equal halves longitudinally, so that two preparations can be prepared. 2.DISSECTION 4.With the help of glass dissecting needle,dissociate the sciatic nerve carefully along edge of the vertebral column,and then looking for the sciatic groove located between biceps femoris and semimembranous muscle, expose and Separate the sciatic nerve down to knee joint longitudinally along the groove and then separate the peroneal nerve located on the surface of gastrocnemius muscle. A thread is tied around the origin of sciatic nerve, cutting the nerve up the thread.Put it in the .shield trough. * * DEMONSTRATE THE GENESIS OF BIPHASIC POTENTIAL A B A B A B Nerve fiber pithing needle great foramen