电针联合嗅鞘细胞移植对大鼠脊髓损伤区脑源性神经营养因子表达的影响-中西医结合临床专业论文.pdf.docxVIP

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电针联合嗅鞘细胞移植对大鼠脊髓损伤区脑源性神经营养因子表达的影响-中西医结合临床专业论文.pdf.docx

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电针联合嗅鞘细胞移植对大鼠脊髓损伤区脑源性神经营养因子表达的影响-中西医结合临床专业论文.pdf

transplantation group is higher than the olfactory ensheathing cell transplantation group, two groups of curative group(P0.05), there is significant difference. 2, BDNF expression changes: 2 weeks build mode between groups have no obvious difference; Olfactory ensheathing cell transplantation group, the electroacupuncture group, electroacupuncture associated with olfactory ensheathing cell transplantation group 3th,5th and 9th weeks while at the same time point in the control group with significant difference(P0.05), and the curative associated with olfactory ensheathing cell transplantation group 3th,5th and 9th weeks, and at the same time point of olfactory ensheathing cell transplantation group, the electric acupuncturegroup with significant difference(P0.05).Conclusion: Electroacupuncture associated with olfactory ensheathing cell transplantation through increased rat spinal cord injury area of the expression of brain derived neurotrophic factor level, so as to promote the injury of the spinal cord repair.Keywords spinal cord injury Olfactory ensheathing cell transplantation; Electroacupuncture; DBNF; BBB scoreAuthor:HaiBo Zhang Tutor:JiHua Liu目录引言···································································1 第一节实验材料·························································2 1 实验动物·························································22 主要试剂·························································23 主要溶液组成及配置···············································24 主要仪器及实验用品···············································35 主要器械及耗材···················································3 第二节实验方法·························································5 1 嗅鞘细胞的原代培养与免疫组化····································52 大鼠 SCI 模型的建立63 动物分组·······················································64 检测指标·······················································7 第三节实验结果·······················································9 1 OECs培养及免疫组化鉴定·······································92 BBB评