dact1和dact3基因在结直肠癌组织中的表达研究word格式论文.docxVIP

AbstractThe Expression of Dact1 and Dact3 Gene in Carcinogenesisof Colorectal TumorBackgroundColorectal carcinoma is one of the most common tumor types in our country. In present, scientists focus their studies on cancer surppressor genes to find out the moleculor mechanisms of tumor oncogenesis. Wnt pathway，which plays an important role in the development of colorectal cancer，is a hot spot of research. On the while, Dact is a key regulator site in the Wnt pathway.The purpose of this study is to observe the methylation of promoter of gene Dact1(Dact3) and the expression of Dact1(Dact3) mRNA in colorectal tumor and to analyze its relationship between them with carcinogenesis of colorectal tumor.Materials and MethodsColorectal cancer tissues 50 pairs of cancerous and corresponding paracancerous were surgically obtained from colorectal cancer patients ，and 20 of normal colorectal tissues were surgically obtained from PPH patients, all of which in the Hangzhou Hospital of anhui Medical University between 2008.1 and 2009.7. All the cancerous tissues had clear pathological diagnosis. The paracancerous were far away about 5cm from cancerous mucoses, and also had clear pathological- diagnosis: normal mucoses cases. All patients were preoperatively end up receiving chemotherapy and otheranti-cancer therapy. All the tissues were stored at -80 ℃forreverse transcriptionpolymerase chain reaction (RT-PCR) and methylation-specific polymerase chain reaction (MSP).RT-PCR:Total RNA was isolated from tissue samples using Trizol Reagent according to the manufacturers protocol. The concentration and purity of total RNA were determinedfrom the A260/A280 ratio using a UV spectrophotometer .One microgram of total RNA from each specimen was subjected to RT-PCR. The β-actin and Dact1(Dact3) PCR products were electrophoresed on a 2% agarose gel and visualized by staining with ethidum bromide. The integrated density values of the bands representing amplified products were acq