dna甲基化作为法医学组织标记的可行性分析word格式论文.docxVIP

（ Xsomatic=82.73%EcoRI, 82.94%TaqI ；Ssomatic=3.54%EcoRI, 3.91%TaqI). When thediscriminant value was set at 49.85%（EcoRIsite）or 50.58%（TaqI site）, semen and non- semen huamn tissues can be discriminated effectively.Conclusion Methylation level of GFAP promoter in brain tissue(including gray and white matter) was significantly lower than that in non-brain tissues, DNA methylation level of GFAP promoter can be used as a brain tissue-specific markers.Methylation level of DDX4 promoter in semen was significantly lower than that in somatic tissue, DNA methylation level of DDX4 promoter can be used as a semen-specific markers.As a proof of concept study, the sample size, methylation marker selected and techniques used here may not be suitable for the forensic practice. However, considering the characteristics of forensic samples and the advantages of DNA markers, tissue specific methylation-variable positions (MVPs) may be ideal tissue makers. Screening for different methylation regions (DMR) among tissues, developing a universally adapted DNA methylation-based tissue ID system may be a solution to the present problems in forensic tissue identification.KEY WORDS: forenisc biology，tissue identification，DNA methylation，GFAP，DDX4Dissertation Type: Applied basic researchIV缩略语词汇表缩略语词汇表GFAP Glial fibrillary acidic protein 胶质纤维酸性蛋白DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 DEAD(Asp-Glu-Ala-Asp)box 多 肽 4COBRA Combined Bisulfite Restriction Analysis 亚硫酸氢钠联合限制性内切酶分 析法STR short tandem repeat 短串联重复序列SNP Single nucleotide polymorphism 单核苷酸多态性T-DMRs tissue-dependent and differentially methylated regions 组织特异性差异甲 基化区域CNS central nervous system 中枢神经系统NPCs neuralprogenitor cells 神经前体细胞JAK/STAT jak(janus actirated kinase)-stat(simal transducers and activators of transcription) JAK/STAT 信号转导通路RLGS restriction landmark genomic scanning 限制性界标基因组扫描GNF Genomics Institute of the Novartis Research Foundation 诺华研究基金会基因 组研究所PCR polymerase chain reaction 聚合酶链式反应BSE Bov