非小细胞肺癌患者介入治疗前后血清hif-1α、vegf的变化及相关性研究word格式论文.docxVIP

英文缩略词表缩略词英文全称中文译名NSCLC non-smallcelllungcancer非小细胞肺癌HIF-1α hypoxia-induciblefactor1alpha缺氧诱导因子-1α VEGFvascularendothelialgrowthfactor血管内皮生长因子ELISAenzymelinkedimmunosorbentassay酶联免疫吸附试验法TGF-βtransforminggrowthfactorbeta-1转化生长因子-β RPMrationperminute每分钟转数非小细胞肺癌患者介入治疗前后血清HIF-1α、VEGF的变化及相关性研究华中科技大学同济医学院附属梨园医院硕士研究生：夏冬平导师：曾玉兰摘要目的：探讨非小细胞肺癌（non-smallcelllungcancer，NSCLC）患者介入治疗前后血清缺氧诱导因子-1α（hypoxia-inducible factor 1 alpha, HIF-1α）和血管内皮生长因子（vascularendothelialgrowthfactor,VEGF）水平的动态变化规律及两者间的相关性。方法：采用酶联免疫吸附试验法（enzyme linked immunosorbent assay,ELISA）检测40 例NSCLC患者介入治疗前、后血清HIF-1α、VEGF水平及30例健康体检者血清HIF-1 α、VEGF水平。结果：1、NSCLC患者血清HIF-1α水平（49.03±8.36）ng/L显著高于正常对照组（37.69±3.33）ng/L（P﹤0.001）；介入治疗前、治疗后1天及7天血清HIF-1α水平分别为：（49.03±8.36）ng/L、（53.46±7.66）ng/L、（60.83±9.43）ng/L，组间比较差异具有统计学意义（P﹤0.02）；2、NSCLC患者血清VEGF水平（742.71±113.09）pg/ml显著高于正常对照组（592.81±71.01）pg/ml（P﹤0.001）；介入治疗前、治疗后1天及7天血清VEGF水平分别为（742.71±113.09）pg/ml、（877.47±164.29）pg/ml、（1119.00±164.27）pg/ml，组间比较差异具有统计学意义（P﹤0.001）；3、介入治疗前、介入治疗后1天及7天血清HIF-1α水平与血清VEGF水平均存在正相关性（r=0.506,P﹤0.01；r=0.406,P﹤0.01；r=0.525,P﹤0.01）。结论：NSCLC患者血清HIF-1α及VEGF水平介入治疗后显著升高，且两者存在显著正相关性。【关键词】非小细胞肺癌；介入治疗；缺氧诱导因子-1α；血管内皮生长因子StudyofthelevelsofserumHIF-1α andVEGFandcorrelationanalysisbeforeandafterinterventionaltherapyforpatients withnon-smallcelllungcancerPostgraduate：XiaDongping Supervisor：ZengYulanAB STRACTObjective：Toinvestigatethedynamicchangeoflevelsofserumhypoxia-induciblefactor1 alpha(HIF-1α)andvascularendothelialgrowthfactor(VEGF)andstudythecorrelationof HIF-1αandVEGFofpatientswithnon-smallcelllungcancerbeforeandafterinterventional therapy.Methods：ThelevelsofserumHIF-1αandVEGFinthe40patientswithNSCLCand30healthyindividuals weredeterminedbyenzymelinkedimmunosorbentassay(ELISA)method.Results:1.ThelevelsofserumHIF-1αinpatientswithNSCLCweresignificantlyhigher thanthoseofthehealthyindividuals(P﹤0.001)；thelevelsofserumHIF-1αontheday beforeinterventionaltherapywere(49.03±8.36)ng/L,thelevelsofserumHIF-1αonthe1stdayand7thdayafterinterv