八氯二丙醚生产工人p53蛋白及氧化损伤检测.docVIP

八氯二丙醚生产工人p53蛋白及氧化损伤检测 【摘要】目的检测八氯二丙醚生产工人氧化损伤及血清p53蛋白表达情况。方法选择暴露于八氯二丙醚工人21名为研究对象，另选21名非暴露者为对照，进行血清p53蛋白及丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的测定。结果暴露于八氯二丙醚工人血清p53蛋白水平略高于对照组，但工龄＞8年组血清p53蛋白的水平显著高于工龄＜8年组，差异有统计学意义(P＜0.01)；SOD、GSH-Px酶活性略低于对照组，MDA水平则显示升高(P＜0.01)。结论长期暴露接触于八氯二丙醚作业工人可有p53基因异常的危险性，以及对氧化性应激的易感性。 【关键词】 八氯二丙醚；氧化损伤；p53蛋白基金项目： 江苏省预防医学基金项目(Y200421)；东南大学科技基金项目(XJ019005) Detection of serum p53 protein and oxidative damage in octachlorodipropylether exposed population TANG Meng,ZENG Chuihuan,WANG Bo,et al. Department of Toxicology,School of Public Health,Southeast University (Nanjing 210009,China) Abstract： Objective Serum p53 protein expression and oxidative damage were detected in population exposed to octachlorodipropyl ether (S2).Methods 21 workers occupationally exposed to S2 were selected as exposed group, and 21 workers without any exposure to chemical toxicants and other hazards served as the control group;p53 protein,MDA,and activities of SOD,GSH-Px in serum were detected in two groups.Results Serum p53 protein expression of the exposure group was significantly higher than that of the control group when stratified by working age;MDA of the exposure group was significantly higher than that of the control group,but the activities of SOD,GSH-Px of the exposure group were lower than those of the control group without statistical significance.Conclusion Exposure to S2 could lead to mutation of p53 gene and oxidative damages. Key words： octachlorodipropyl ether;oxidative damage;p53 protein 八氯二丙醚(Octachlorodipropyl ether，简称S2或S421)。八氯二丙醚作为农药的优良增效剂，可以明显提高拟除虫菊酯和氨基甲酸酯类药物的杀虫效果〔１〕。p53蛋白是p53抑癌基因表达产物，对调控细胞增殖周期、细胞凋亡起重要作用。已发现多种肿瘤存在p53基因异常表达。有研究发现，抑癌基因与职业性肺癌密切相关，其改变可发生在癌变的早期阶段，并于血清中出现相应的蛋白产物〔２〕。八氯二丙醚生产职业接触者有小细胞肺癌发生的报道，且有实验结果提示，其生产工人外周血检测到DNA损伤，而DNA受到损伤时可反馈性的激发p53基因的表达，引起细胞内野生型p53蛋白的表达增加〔３〕。为了解八氯二丙醚职业接触人群血清p53蛋白表达情况及生产工人氧化损伤情况，进行本研究。结果报告如下。 1 对象与方法 1.1 对象 选择江苏某八氯二丙醚生产厂全部21名工人为暴露组，平均年龄为(43.38±6.82)岁；另选当地一个绳网厂21名职工作为对照组，平均年龄为(41.24±8.65)岁。被调查对象均为男性，均衡可比。 1.2 检测标本 每名被调查者从