大鼠肝缺血再灌注损伤时卵巢机能代谢指标的变化及其意义.docVIP

大鼠肝缺血再灌注损伤时卵巢机能代谢指标的变化及其意义 【摘要】 目的：探讨大鼠肝缺血再灌注（I/R）损伤过程中卵巢组织能量代谢的变化及可能的发生机制。方法：健康雌性Wistar大鼠48只随机分为对照组、缺血30 min组（Ⅰ组）及缺血30 min再灌注即刻组、2 h、4 h、6 h组（I/R及I/R 2、4、6 h组），每组8只,检测各组卵巢组织匀浆中髓过氧化物酶（MPO）、乳酸脱氢酶（LDH）的活性及丙二醛（MDA）、乳酸（LD）的含量，采用放射免疫法检测血清中雌二醇、孕酮含量。结果：肝I/R损伤中，I/R、I/R 2 h组卵巢组织MPO的活性明显低于I、I/R 6 h组,差异有统计学意义（P＜0.05）,I/R、I/R 2 h组卵巢组织中MDＡ及血清雌二醇、孕酮含量与I/R 4 h、I/R 6 h组比较差异有统计学意义（P＜0.05），卵巢组织中MPO与MDA呈正相关（r=0.814，P＜0.05），I/R、I/R 2 h组LDH活性明显高于对照组、I组，差异有统计学意义（P＜0.05)，I/R 2、4、6 h组卵巢组织中LD的含量明显高于I/R组，差异有统计学意义（P＜0.05)。 结论：肝I/R损伤初期卵巢分泌雌、孕激素代偿性升高可能对机体产生一定保护作用，但随着再灌注时间的延长仍可引起卵巢组织明显的能量代谢障碍。肝I/R损伤过程中，卵巢组织中MPO介导的氧化应激反应可能参与了卵巢组织损伤的发生。 【关键词】 缺血再灌注　肝脏　卵巢　髓过氧化物酶　乳酸脱氢酶　乳酸　雌二醇　 孕酮 The changes and significance of the energy mechanism in ovary during liver ischemia and reperfusion injury in rats JIANG Wenjuan, WANG Hongmei, ZHANG Jianlong, et al 1Medical Emergency Center of Urumqi, 2Department of Pathophysiology, Xjnjiang Medical University, Urumqi 830000, China Abstract：Objective: The changes of myeloperoxidase(MPO) histology and morphology in liver ischemiareperfusion (I/R) injury were observed, the relationship with the energy metabolism in ovarian were investigated and its mechanism were studied following the process of liver I/R in rats. Methods: A model of liver I/R injury was established by imitation. Forty eight female Wistar rats were randomly divided into 6 groups as following (n=8): the control group (CTL), the group of sham operateration, simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 2 hour (I/R 2 h), 4 hours (I/R4 h) and 6 hours (I/R6 h) group. The changes of MPO and MDA in ovarian and the changes lactic dehydrogenase (LDH), lactic acid(LD) were measured. Radioimmunoassay (RIA) was used to determine the serumal levels of estradiol and progesterone. Results: During the process of liver I/R. The level of MPO had obvious statistical significant difference in I/R, I/R 2 h group compared with I, I/R 6 h （P＜0.05). The level of MDA,