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截短侧耳素发酵工艺优化-optimization of fermentation technology of pleuromutilin
2.6.2发酵培养基响应面优化方法......................................122.7补料方法的优化试验..............................................132.7.1补料试验方法..................................................133实验结果及分析.....................................................143.1种子培养基优化试验结果..........................................143.1.1种子培养基单因素试验结果......................................143.1.2种子培养基正交试验............................................193.2发酵培养基优化..................................................223.2.1发酵培养基单因素实验结果......................................223.2.2发酵培养基响应面优化结果......................................263.4补料优化实验结果................................................313.4.1两种补料方法对PH的影响313.4.2两种补料方法对菌浓的影响......................................323.4.3两种补料方法对截短侧耳素产量的影响............................334结论...............................................................335展望...............................................................34致谢..............................................................35参考文献........................................................36作者简介........................................................39插图和附表清单1.图1截短侧图表耳素的结构.....................................................12.图2从截短侧耳素的合成途径...................................................23.图3泰妙菌素的结构...........................................................34.图4沃尼妙林的结构...........................................................45.图5瑞他莫林的结构...........................................................56.图6种子菌浓曲线............................................................147.图7不同葡萄糖浓度对产量的影响..............................................158.图8不同豆饼粉浓度对发酵产量的影响..........................................159.图9不同KH2PO4浓度对发酵产量的影响1610.图10不同Ca(NO3)2浓度对发酵产量的影响1611.图11不同MgSO4浓度对发酵产量的影响1712.图12不同NaCl浓度对发酵产量的影响1713.图13不同浓度FeSO4对发酵产量的影响1814.图14种子培养基方差分析效应曲面图..........................................2015.图15优化后种子浓度变化....................................................2016.图16种龄对产量的影响......................................................2117.图17接
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