利用双t-using double t.docxVIP

2.4 讨论 .................................................................................................................. 19第 3 章红阳猕猴桃农杆菌介导遗传转化体系的建立................................ 213.1 实验材料 .......................................................................................................... 213.1.1 材料与仪器 ........................................................................................... 213.1.2 培养基 ................................................................................................... 223.2 农杆菌介导的愈伤转化体系 .......................................................................... 233.2.1 红阳猕猴桃茎段愈伤组织的诱导 ....................................................... 233.2.2 红阳猕猴桃叶片愈伤组织的诱导 ....................................................... 233.2.3 愈伤组织继代 ....................................................................................... 233.2.4 愈伤组织分化 ....................................................................................... 233.2.5 农杆菌的准备与愈伤组织预培养 ....................................................... 233.2.6 共培养 ................................................................................................... 243.2.7 抗性愈伤组织的筛选 ........................................................................... 243.2.8 抗性愈伤组织的分化 ........................................................................... 243.2.9 抗性植株的生根及练苗 ....................................................................... 243.3 叶盘转化法 ...................................................................................................... 253.4 结果和分析 ...................................................................................................... 253.5 讨论 .................................................................................................................. 29第 4 章抗性植株的 PCR 检测 304.1 实验材料 .......................................................................................................... 304.2 实验方法 ....................................................