荔枝 龙眼离体培养及再生植株的分析-analysis on in vitro culture and plantlet regeneration of litchi and longan.docxVIP

诱导芽苗生根较适合的培养基分别为MS+0.05mg/L6-BA+1.0mg/LIBA+3%蔗糖+0.7%琼脂、MS+0.1mg/L6-BA+1.0mg/LIBA+3%蔗糖+0.7%琼脂，生根率分别为2.9%、5.0%。6、以石硖龙眼种子为外植体，通过离体培养体胚发生方式初步获得了再生苗，但没有生根，其生根诱导还需进一步研究。关键词：荔枝；龙眼；离体培养；胚性愈伤组织；体胚分化；植株再生TheStudyoftheVitroCultureandRegenerationPlantofLitchiandLonganGuoShaoyun（CollegeofAgriculture,GuangxiUniversity,Nanning530004,China）AbstractLitchiandlonganaredifficultinvitroculture,andtherateofthenormalsomaticembryoinductionandregenerationplantislow,whichhaverestrictedtheprocessofnewvarietybreedingofthemanddevelopmentofthebiotechnology.TheshixialonganandHinensislitchiinguangxiweretakenastestmaterials,mainlystudiedtheprocessoftheanther,stem,bladeandseedastheexplantsinvitrocultureinducingtheoccurrenceofcallus,whichhadbasicallyestablishedtheregenerationsystemoflizhi;Andpreliminaryexploredtheprocessofsomaticembryogenesisandplantregenerationbytheisolatedcultureoflonganseeds.Moreover,thestemsegmentsofthemweretakenastheexplants,whichhadrespectivelygottentheregenerationplantbythewayoftheorganogenesis,thisresulthadprovidedtheoreticalbasisandtechnicalsupportfortheresearchofthebasictheoryofthebiologicaltechnologyandthegenetictransformationandthenewvarietybreedingofthem.Theresultswereshowedasfollows:Theprocessoftheanther,leaf,stemandseedastheexplantsinvitrocultureinducingtheoccurrenceofcallushadgottenthedifferenttypesofcallus,thehighestinductionratewere82.1%,86.0%,81.3%and84.0%respectively.Themediaofbettersuitableforformationofembryoniccallusofthelitchiantherwas:MS+0.1g/LVC+2.5mg/L2,4-D+0.5mg/L6-BA+1.0mg/LNAA+2.0mg/LKT+3%sucrose+0.7%agar.Theculturemediumofthesubcultureoftheembryoniccallusinducedbytheantherwas:MS+1.5mg/L2,4-D+0.5mg/L6-BA+3%sucrose+0.7%agarandMS+1.0mg/L2,4-D+1.0mg/LKT+5.0mg/LAgNO3+3%sucrose+0.7%agar,bythewayofthealternatelysuccessivetransferculturetogetthestablelinesoftheembryoniccallus,andsuccessivetransfercultivatedonce21d,successivetransfercultivated4-5times.Thehigh-frequencyculturemediumofthesomaticembryowas:MS+100mg/Linositol+0.1mg/LNAA+3.0mg/LCPPU+80mlcoconutmilk/L+