荔枝采后主要病原菌的特性及内生细菌对其防治保鲜分析-characteristics of main pathogens in litchi postharvest and analysis of endophytic bacteria on their control and preservation.docxVIP

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2018-05-29 发布于上海
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荔枝采后主要病原菌的特性及内生细菌对其防治保鲜分析-characteristics of main pathogens in litchi postharvest and analysis of endophytic bacteria on their control and preservation.docx

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荔枝采后主要病原菌的特性及内生细菌对其防治保鲜分析-characteristics of main pathogens in litchi postharvest and analysis of endophytic bacteria on their control and preservation

AbstractLitchichinensisisafamoussubtropicalfruitinthesouthernChina,alsoisoneofthemostintolerantstoragefruit.Pathogeninfectionandfruitbrowningwerethemainreasonsaffectinginitsstorageperiod.Presently,chemicalswerecommonlyusedincontrollingthisdisease.Buttherearemoreshortcomings,suchaspesticideresidues,environmentalpollutionandsoon.Sothatbiologicalcontrolhasbecamethehotspotstudy.Otherwise,thevegetativeofOomyceteisdiploid.TraditionalmethodofgenemutationsuchasknockoutwasnotfeasibletouseinOomycetes.TransformingendogenesisgeneandcausingitssilencearethemosttechniquetostudythegenefunctionofOomycetes.Inpresent,thereisalackofefficientmoleculegeneticmanipulation,andthestudyonmoleculelevelissoslow.Therefore,theBiologicalcharacteristicsoflycheepost-harvestdiseasepathogens,transformationsystem,thecontrolorpreservationbyendophyticbacteriaweresystemiclystudiedinthispaper.Themainrusultsareasfollows.BiologicalcharacteristicsofPeronophthoralitchii.ThebiologicalcharacteristicsofP.litchiidemonstratedthatthegrowthofitssporangiophorebelongstomulti-determinativetype.Thesporangiacouldgerminatetoformvariousshapes,zoospores,tubes,sporangiophore,orsecondarysporangia.Thegerminatingholesmightlocateintheoneside,thebothsidesorroundnessofthesporangia.Inoneholeoneormoreradicalbudsmightgerminateout.Thebaseholesofthesporangiausuallyareeasiertogerminateandcouldformchlamydosporesandoosporesafterculturingfor35and45drespectively.Thesporangiaproducedfromthefungusculturedfor10dwouldhavethebestgerminatingrate.Thesporangiagerminatedtoformzoosporesat16~24℃andtubesat26℃.ThemoresporeswereproducedonPDAplate,andthefungusgrowsfastestontheoatplate.ThemostsuitablepHforitsgrowthis6~8;Inthesametime,Theinfluencingfactorsonthemycelialgrowth,sporulation,sporangiagerminationandinfectionofP.litchtiiweresystemiclystudied.TheresultshowedthattheoptimumconditionsforthemyceliagrowthandsporulationofP.litchiiwereinthemediumofpH7.0andat24℃.Theoptimumilluminationforthemycelialgrowthandsporulationofthefunguswereunderlightfor20hand16hsep