《超分辨成像技术》细胞成像技术小组展示.pptxVIP

超分辨成像技术;Abbe’s diffraction limit;光学显微镜的分辨率公式;光的波长：??;物方折射率：??;孔径角：??;NSOM(Near-field scanning optical microscope);NSOM探测原理;Apertured Mode;NSOM优缺点;NSOM应用;NSOM应用;NSOM应用;基于单分子定位成像： STORM PALM ;The principle of single-molecule microscopy ;STORM(stochastic optical reconstruction microscopy);PALM(photoactivated localization microscopy);PALM STORM 优缺点;Distribution of GFP-labeled lysosome protein (CD63) without (A) and with (B) PALM. Large box in B at higher magnification (C).Small box in B at higher magnification (D). Note that the scale bar in D is close to Abbe’s limit;Fluorescence images of mitochondria (magenta) and microtubules (green) observed by (a) conventional wide-field microscopy and (b)STORM. Scale bar = 3 μm.;Two-color STORM imaging of microtubules and CCPs in a mammalian cell. (A) STORM image of a large area of a BS-C-1 cell. The secondary antibodies used for microtubule staining were labeled with Cy2 and Alexa 647, while those for clathrin were labeled with Cy3 and Alexa 647. The 457 nm and 532 nm laser pulses were used to selectively activate the two pairs. Each localization was colored according to the following code: green for 457nm activation and red for 532 nm activation. (B) STORM image corresponding to the boxed region in (A) shown at a higher magnification. (C) Further magnified view of the boxed region in (B).;STED(Stimulated emission depletion microscopy);RESOLFTreversible saturable (or switchable) optical fluorescence transitions ;STED优缺点;STED应用;STED应用;STED应用;SIM(Structured Illumination Microscopy);SIM优缺点;SIM应用;3D-SIM;Thank you for your attention