六甲基杨梅素的合成及其对hkv1.5通道的影响与国产fgf-synthesis of hexamethylmyricetin and its influence on hkv 1.5 channel and domestic fgf.docxVIP

Result:10μMverapamildecreasedultrarapidactiveddelayedrectifiedpotassiumcurrentby87±5%,andthiseffectcouldbepartiallyreversedbywashoutverapamil,whichprovedthecurrentrecordedwasIKur,whichwasinhibitedbytheapplicationof0.1、0.3、1、3μMHMMandwaspartiallyreversedbywashoutHMM.Statistically,3μMHMMcansignificantlysuppressIKur.TheIC50ofHMMeffectonIKuris1.79μM.Conclusion:HexamethylmyricetinsignificantlyinhibitedhKv1.5channelcurrents.Keywords:hKv1.5channel;IKur;atrialfibrillation.PartⅢTheeffectsofFGF-21manufactureredbyGROSTonKKAyandob/obdiabeticmiceAim:ToinvestigatetheeffectsofFGF-21manufactureredbyGROSTonKKAyandob/obdiabeticmice.Methods:①KKAymicewerehousedindividuallyincages,whichwerefedahigh-fatdietadlibitum,while,ob/obmicewerehousedincages,eachcagecontains5mice,whichwerefedanormalchowdietadlibitum.BothKKAyandob/obmicewerelocatedinaroomwithcontrolledtemperature(23±2℃),humidity50%,lightedonfrom7:00-19:00andwereallowedwateradlibitum.②BloodwascollectedfromthetoeofKKAyandob/obmicebyeyescissors,whenthevolumeofbleedingisabout0.6μl,bloodglucosewasproceedbyRocheglucometer,thevalueandthetimewererecorded.③oralglucosetolerancetest(OGTT,oralglucosetolerancetest).ob/obmicewerefastingfor2h,collected0minbloodglucoseascontrolstandard,thenoraladministration2g/kgglucosesolution,bloodsamplesweretakenafteradministration2g/kgglucosesolution30min,60minand120min.Results:①FGF-21shownoobviouseffectonKKAymice;②FGF-21low-dose(0.6mg/kg/d)candecreasefastingbloodglucoseonmaleob/obmice,though，thedifferencewasnotstatisticalsignificant;FGF-21highdose(2.5mg/kg/d)havealoweringpostprandialbloodglucoseeffectonmaleob/obmice,whichonthe7thdayafteradministration,thedifferencewasstatisticalsignificant;③AboutOGTTresults,FGF-21low-dose(0.6mg/kg/d)haveacertainroleintheimprovementofimpairedglucosetoleranceonmaleob/obmice.Conclusion:FGF-21displayacertainroleintheanti-diabetesactionwhichasbelow:reducebloodglucoselevelonmaleob/obmice,correctinsulinresistance.Therefore,FGF-21islikelytobeausefulclinicaldrugtopreventandtreatdiabet