kai1基因对mhcc97h肝癌细胞侵袭转移的影响及机制-effect and mechanism of kai 1 gene on invasion and metastasis of mhcc 97 h hepatocarcinoma cells.docxVIP

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kai1基因对mhcc97h肝癌细胞侵袭转移的影响及机制-effect and mechanism of kai 1 gene on invasion and metastasis of mhcc 97 h hepatocarcinoma cells.docx

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kai1基因对mhcc97h肝癌细胞侵袭转移的影响及机制-effect and mechanism of kai 1 gene on invasion and metastasis of mhcc 97 h hepatocarcinoma cells

第三军医大学硕士学位论文第三军医大学硕士学位论文 PAGE PAGE 1 英文缩写一览表英文缩写 DMEM 英文全称 Dulbeccos minimum essential 中文全称 Dulbeccos 极限必需培养基 DMSO (or Modified Eagle) medium Dimethyl sulfoxide 二甲基亚砜 EDTA HCC Kb PBS TM4SF DAB BSA FN LN ECM BM Col IV ICAM-1 HE Ethylenediaminetetraacetic acid Hepatocellular carcinoma Kilo-base pair Phosphate-buffered saline Transmembrane 4 superfamily 3,3’ -Diamionbenzidene Bovine serum albumin Fibronectin Laminin Extrancellularmatri Basement membrane Collagen IV Intercellular adhesion molecule Hemataoxylin and eosin 乙二胺四乙酸肝细胞肝癌千碱基对磷酸缓冲盐水跨膜 4 超家族 3,3’ -二氨基联苯胺牛血清白蛋白纤维连接蛋白层粘连蛋白细胞外基质基底膜型胶原细胞间黏附分子-1 苏木素和伊红 AFP ECM -fetal protein Extrancellularmatri 甲胎蛋白细胞外基质 Effects and mechanisms of KAI1 gene on invasion and metastasis of MHCC97-H human hepatocellular carcinoma cells ABSTRACT Background and aims:Hepatocellular carcinoma(HCC) is one of the most common malignant tumors in China.Metastasis and recurrence are the most principal factors for their prognosis of the patients with the tumor.KAI1 gene is a newly found tumor metastasis suppressor gene.Many studies showed decreased KAI1 expression was observed not only in human prostate cancer but also in many solid epithelial tumors.The aim of this study is to explore the effects and mechanisms of KAI1 gene on invasion and metastasis of MHCC97-H human hepatocellular carcinoma cells. Methods: MHCC97-H cells with high metastatic potential transfected with sense, antisense KAI1 expression plasmid or its control vector pCI-neo without KAI1 gene in our previous experiments are renamed as MHCC97-H-S cells, MHCC97-H-AS cells, MHCC97-H-pCI cells respectively.In this study,we observed their viscoelastic properties,cell adhesion forces, subcutaneous tumorigenicity and metastasis after implanted orthotopicly into nude mice liver,and the expression of ECM and intercellular adhesion mollecular. Results:(1)Cell viscoelastic properties: The elastic coefficients K1 K2 and of the MHCC97-H cells were significantly higher after tran