中西药结合治疗CLP所致大鼠SIRS实验研究.docVIP

中西药结合治疗CLP所致大鼠SIRS实验研究 　　摘 要：目的: 观察抗生素联合中药治疗对CLP所致大鼠SIRS的保护作用。方法:采用盲肠结扎穿孔术（Cecal ligation and punctured,CLP）制备大鼠SIRS模型, 与空白及治疗对照组相比较。观察大鼠15min～48h 的行为活动、精神状态、食欲、呼吸、大小便及死亡率等一般情况；于造模后12h眶静脉取血测白细胞、红细胞、血小板计数及TNF－α浓度的变化。结果: 治疗组与模型组大鼠均出现一般情况恶化;各治疗组死亡率均低于模型组，而中药＋抗生素对照组降低最明显；模型组与空白对照组比较：WBC 、TNF－α较高(P 　　 　　关键词：全身炎症反应综合征（SIRS）；死亡率；TNF－a 　　中图分类号：R285.5 　　文献标识码：A文章编号： 　　1673-7717（2008）05-0932-03 　　?? 　　Experimental Study of Combining Antibiotics? 　　with Traditional Chinese Medicine in Treatment of SIRS Rats Induced by CLP. 　　?? 　　JIANG Shu?min?1,WU Dong?mei?2,GU Li?fu?2,SUN Ke?feng?2 ?（ 　　1.Emergency Center, Liaoning Traditional Chinese Medical Hospital, Shenyang 110032,Liaoning,China; ? 　　2.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning, China）? 　　Abstract：Objective:To investigate the protection effect of the antibiotic combining tradotion 　　Chinesemedicine in treatment of SIRS rats induced by CLP. Methods: Cecal ligrarition and puncture to make the model of sepsis in rats SIRS and compare with blank and treatment contrast group. Observe the general conditions such as mortality, state of mind, appetite, respiration, natural necessity and the behavior campaign of big mouse 15min～48h; The 12h after makes model 　　Kuang vein takes blood to measureleucocyte, red blood cell and blood platelet count and TNF- α the change of density. Results: Treating group and model to organize big mouse arise general condition deterioration; Each treatment group mortality is organized below model, traditional Chinese medicine＋antibiotic contrast group reduction is most obvious，Model group compares with blank contrast group: WBC and TNF- α is higher ( P 　　1.4实验分组 随机分为8组：①空白对照组（8只）NS 20mL/（kg#8226;d），日2次ig；②假手术对照组（8只）开腹后仅作盲肠翻动，NS 20mL/（kg#8226;d），日2次ig；③模型对照组（16只）盲肠结扎穿孔制???SIRS模型，NS 20mL/（kg#8226;d）日2次ig；④解毒抗炎合剂低剂量组（17只）盲肠结扎穿孔制作SIRS模型，术后立即给予解毒抗炎合剂10g/（kg#8226;d），日2次ig；⑤解毒抗炎合剂高剂量组（17只）盲肠结扎穿孔制作SIRS模型，术后立即给予解毒抗炎合剂30g/（kg#8226;d），日2次ig；⑥抗生素对照组（15只）盲