胚胎晚期母体暴露细菌脂多糖加速cd 1小鼠海马依赖性行为年龄相关性改变-maternal exposure to bacterial lipopolysaccharide in late embryo accelerates age-related changes of hippocampal dependent behaviors in cd 1 mice.docxVIP

统计学差异（F(1,16)=0.762,P=0.391），但雄鼠体重大于雌鼠（F(1,16)=27.20,P0.01）；②在35日龄时，LPS组和对照组间的焦虑行为和自发探索活动能力（旷场任务）差异无统计学意义（P＞0.05）；③在290日龄时，LPS组和对照组间的种属行为（储藏、挖掘、筑巢）、焦虑行为和自发探索活动能力（旷场任务）、感觉平衡运动能力（10mm圆平衡木和紧绳任务）及空间学习记忆能力（六臂辐射状水迷宫任务）差异无统计学意义（P＞0.05）；④在400日龄时，LPS组和对照组之间的自发探索活动能力和焦虑行为（旷场、高架十字迷宫、黑白巷任务）、感觉平衡运动能力（平衡木和紧绳任务）差异无统计学意义（P＞0.05）。但是，LPS组小鼠储藏和挖掘能力高于对照组（P＜0.05），而筑巢能力低于对照组（P＜0.05）。LPS组的空间学习记忆能力（Morris水迷宫任务）和非空间学习记忆能力（新物体再认任务）低于对照组（P＜0.05）。结论胚胎晚期母体暴露LPS对CD-1小鼠的生长发育及神经系统成熟没有影响，但加速子代以海马依赖性种属行为及空间和非空间性学习记忆能力的衰退。关键词衰老；小鼠；学习和记忆；脂多糖MaternalExposuretoLipopolysaccharideinLateEmbryoAcceleratesAge-RelatedChangesofHippocampal-DependentNeurobehaviorsinCD-1MiceABSTRACTBackgroundThe“developmentaloriginsofadultdisease”theoryhasbeensupportedbyincreasingbodyofevience.Thecoreofthistheoryisthatmanyadultdiseasesresultfromtheunhealthyfactorsexposedinearlylifeofhumans,suchasembryonicorneonatalperiod.Exposuretoadverseenvironmentalfactors,suchasdystrophy,stress,chemicaltoxicantsandsoon,cancauseapersistentprogrammingchangeofdevelopmentalbodystructure,physiologyandmetabolism.Therefore,neurodegenerativediseases,e.g.,Alzheimer’sdisease,Parkinson’sdisease,maybederivedfrominsultsoccurredintheearlyhumanlife.AnimalexperimentalresearcheshavefoundthatprenatalexposuretoLipopolysaccharides(LPS),thetoxicalcomponentofthecellwallofGram-negativebacteria,cancauseneonataloradultoffspringneurodevelopmentaldamages.RecentresearchesshowthatLPSexposureinthemid-or-latestageembryoscanleadtodeclineabilityoflearningandmemoryinadultfull-termoffspringmice.However,thereisnoreportaboutthedynamicevaluationofgrowth-developmentandsystematicneurobehaviorsinlife-timefromweantomiddle-agedintheoffspringwhosemotherexposestoLPSduringpregnancy.ObjectiveToinvestigatetheeffectsofmaternalexposedtoLPSinlateembryoongrowth-developmentanddynamicneurobehaviorsintheCD-1offspringwhosemotherexposestoLPSduringpregnancy.MethodsCD-1mice(atageof7-8weeks;malemice:30–32g;femalemice:28–30g)werefedforacclimatizationbeforeexperiment.Thefemalesandmaleswerematedovernighta