原发肾病综合征患者尿白细胞介素-18及肾损伤因子-1检测及其意义-detection and significance of urinary interleukin - 18 and renal injury factor - 1 in patients with primary nephrotic syndrome.docxVIP

ThesignificanceofurineInterluekin-18andKidneyInjureMolecule-1inpatientswithPrimaryNephropathySyndromeABSTRACTObjective:Primarynephroticsyndrome(PNS)isthecommonrenaldisease,acuterenalfailure(ARF)isoneofthecommonandseriouscomplications.Acutetubularnecrosis(ATN)istheprimarypathologicalfeatureinthepatientsofPNScomplicatedwithARF,andATNwassignificantlyupregulatedpriortotheincreaseinserumcreatinineinsomepatientsofPNScomplicatedwithARF.RenalbiopsypunctureistheclinicalgoldstandardtodiagnosetheATN,butitisofdestructiveness,chancinessandlimitations.Sotryingtofindmoreconvenient,specificandsensitivebiologicfactorstohelpthediagnosisofPNScomplicatedwithATNisessentialandofsignificantclinicalvalue.Accordingtothestudies,manyurinemoleculesrelatedtoacutekidneyinjure(AKI)werefound,Interleukin-18(IL-18)andkidneyinjurymolecule-1(KIM-1)weretwooftheimportantcytokines.OurstudyistoinvestigatetheurinarylevelofIL-18andKIM-1(byenzymelinkedimmunosorbentassay)inthepatientsofPNS,andtocarryouttherelativeresearcheswithtrialindexcommonlyusedinclinicalindex(suchas24hurineprotein,serumcreatinine,tubulointerstitiallesions),soastodiscussthepossibleeffectofIL-18andKIM-1inthePNScomplicatedwithARF.Methods:Step1:Weselected74patientsasthecases(44men,30women),whoconformedtothecriterionofprimarynephroticsyndrome(24hurineprotein3.5g,plasmaalbumin30g/L).Theyweredividedintofourgroups：1.Minimalchangedisease(MCD)(18menand15women);2.Membranousnephropathy(MN)(15menand12women,18StageIMNcases,7StageIIMN,2StageⅢMN);3.Focalsegmentalglomerulosclerosis(FSGS)(4menand2women,4non-special-typefsgscases,2top-typefsgscases),4.mesangialproliferativeglomerulonephritis(MsPGN)group(5menand3women).MCDgroupweredividedintotwogroups(complicatedwithATNgroupandnocomplicatedwithATNgroup).Step2:15cases(8menand7women)werecollectedfromthepatientswhowereperformednormalurineanalysisinthehealthexamination.Step3:Enzymelinkedimmunosorbentassay(ELISA)wasappliedtodetecttheurinarylevelofIL-18andKIM-1inPNSpatientsandthecontrolgroup.Alsocomparationswerem