原油对日本大螯蜚grandidierella japonica的毒性效应分析-analysis of toxic effects of crude oil on japans great chelator grandidierella japonica.docxVIP

AbstractGrandidierella japonica, being to Arthropoda, was selected to as the testing organism for analyzing the acute and chronic damages of spilled oil on sea and marine organisms. The effects of heavy and light oil Water-soluble fractions (WAF) on survival of G. japonica individual and activity of biomarkers Malondialdehyde (MDA), Reduced glutathione (GSH), Glutathione peroxidase (GSH-Px), and Glutathione S-transferase (GST) were studied. The results were analyzed and assessed basing on the integrated biomarker responses.The results of 96h acute toxicity test: increasing concentration of heavy and light oil WSF versus increasing mortalities of G. japonica individual at 24, 48, 72, 96 testing hours. The half lethal concentration of heavy and light oil WSF are 1.74mg·L-1 and 2.09 mg·L-1, respectively.The results of 7d chronic toxicity test: different level effects of heavy and light oil WSF on 5 biomarkers of G. japonica in vivo.MDA content increased in accordance with the increase of heavy oil WSF concentrations. Compared with MDA content in the control group, there was no significant difference in the lowest concentration group (p0.05), while there was a highly obvious difference in two high concentration group (p0.01). Exposure to light oil WSF, MDA content showed a climb up and then decline u- typed trend. Maximum value of MDAcontent appeared in the group of 121μg·L-1, and there was a highly significant differencecompared with the control group (p0.01). MDA content began to descend from the group of 363μg·L-1 to 1087μg·L-1and there was a significant (p0.05) and highly significant difference (p0.01) compared with the control group. The highest concentration group, MDA content had no significant difference to the comparison.The contents of GSH of G. japonica , in four test groups , increased at first and then descended with the increasing concentration of heavy oil WSF, but had a less decrease as compared with the control group (p0.05). Similarly, they have a same