乌司他丁对胃癌细胞c-met基因表达及增殖影响研究.docVIP

乌司他丁对胃癌细胞c-met基因表达及增殖影响研究 　　[摘要] 目的：观察乌司他丁对胃癌细胞c-met基因表达及增殖的影响，为乌司他丁用于胃癌的治疗提供理论依据。方法：取对数生长期人胃癌BGC-823细胞，随机分为空白组、对照组和乌司他丁组。空白组加入RPMI 1640培养液3 ml，对照组培养液中加入奥沙利铂(L-OHP)至终浓度为1 mmol/L(对照1组)和0.5 mmol/L(对照2组)，乌司他丁组培养液中加入乌司他丁至终浓度为320 U/ml(乌司他丁1组)和160 U/ml(乌司他丁2组)，分别置于培养箱中培养48 h。采用RT-PCR检测胃癌细胞c-met mRNA的表达情况；ELISA检测细胞抑制率；活细胞计数计算细胞倍增时间，以评估乌司他丁对胃癌BGC-823细胞增殖的抑制情况。结果：乌司他丁组与空白组及对照组比较，c-met mRNA的表达均有显著性差异(P 　　[关键词] 乌司他丁；胃癌；c-met；增殖 　　[中图分类号] R735.2[文献标识码]A [文章编号]1673-7210（2008）11（a）-012-03 　　 　　The influence of Ulinastatin on c-met expression and proliferation of gastric carcinoma cell line BGC-823 cells 　　CUI Ning 　　(Department of General Surgery, the First Peoples Hospital of Shangqiu, Henan Province, Shangqiu476000, China) 　　[Abstract] Objective: To investigate the influence of Ulinastatin on c-met expression and proliferation of gastric carcinoma cell line BGC-823 cells, and provide a theoretical basis for the treatment of gastric carcinoma with Ulinastatin. Methods: Human gastric carcinoma cell line BGC-823 cells in logarithmic growth phase were randomly divided into five groups: blank group, control group 1 and 2, Ulinastatin group 1 and 2. Cells in blank group were cultivated with RPMI 1640 medium. Oxaliplatin (L-OHP) was added into the medium of control group 1 and 2 to make the final concentrations reach 1 mmol/L and 0.5 mmol/L respectively. Then Ulinastatin was added into the medium of Ulinastatin group 1 and 2 to make the final concentrations reach 320 U/ml and 160 U/ml respectively. RT-PCR was employed to detect the c-met mRNA expression 48 h after the culture, and ELISA was used to detect the cell inhibition(CI), and viable cell count was used to calculate the cell doubling time. Results: The c-met mRNA expression of human gastric carcinoma cell line BGC-823 cells showed significant differences among Ulinastatin, control group and blank group(P 　　1 材料与方法 　　1.1 材料 　　1.1.1 细胞人胃癌细胞株BGC-823细胞由中科院上海细胞生物所细胞库提供。 　　1.1.2 主要试剂奥沙利铂(L-OHP，江苏恒瑞医药股份有限公司)，乌