匀浆法提取、离子色谱法测定烟草中糖.docVIP

匀浆法提取、离子色谱法测定烟草中糖 　　摘要:研究了用匀浆法提取、离子色谱法测定烟草中的糖.烟草样品用水匀浆法提取；提取液中的糖以 CarboPac PA1 (4×250-mm，5-μm) 阴离子交换柱为固定相，0.2-mol/L氢氧化钠溶液为流动相分离，脉冲安培检测器检测；1次进样可同时测定烟草样品中葡萄糖、果糖、蔗糖.方法加标回收率在96％~103%之间，相对标准偏差均小于2.6%.检测限均小于50-μg/L，结果满意. 　　关键词:离子色谱法；匀浆法提取；烟草；糖 　　中图分类号:O658 　　 文献标识码:A文章编号:1672-8513(2010)01-0043-03 　　Determination of Sugars in Tobacco by Ion Chromatography 　　and Sample Preparation with High-Speed Homogenization 　　XU Yi?ran1, LI Hai?yan1,3, LI Yin?ke1,2, HE Shun?qin1,2, HU Qiu?fen2 　　(1. Key Lab of Tobacco Chemistry of Yunnan Province, Yunnan Academy of Tobacco Science, Kunming 650106, China; 　　2. School of Chemistry and Biotechnology, Yunnan University of Nationalities, Kunming 650031, China; 　　3.Yunnan Tobacco Quality Supervision and Inspection Station, Kunming 650106, China) 　　Abstract: 　　 A new method for the determination of sugars (fructose, glucose and sucrose) in tobacco by ion chromatography and sample preparation with high-speed homogenization was tested. The sugars were extracted from the samples by high-speed homogenization with water, and the fructose, glucose and sucrose were analyzed by ion chromatography with the integrated pulsed amperometric detector. The three components were separated on CarboPac PA1 (4×250 mm，5μm) anion exchange column by using 0.2 mol/L sodium hydroxide solution as the mobile phase. The limits of detections (S/N=3) were below 50 μg/L. The mean recoveries range from 96％~103%, and the relative standard deviations are below 2.6%.This method has been successfully applied to the determination of fructose, glucose and sucrose in tobacco with good results.. 　　Key words: 　　ion chromatography; high-speed homogenization; tobacco; sugar 　　0 引言 　　糖是烟草中的重要成分，烟草样品中含有多种糖，但含量最高的是葡萄糖、果糖和蔗糖，3种糖占烟草中糖总量的90％以上；烟草中糖的含量和烟草品质有密切的相关性，因此烟草中糖的测定已逐渐成为国内外烟草行业的质量控制内容 ［1-2］；目前，糖的测定方法主要有滴定法、近红外分光光度???、连续流动分析法、毛细管电泳法、气相色谱法、高效液相色谱法和离子色谱法等.样品前处理一般采用回流提取，振荡浸取、超声浸取等方法［2-8］.匀浆提取是指组织通过加入萃取溶剂进行组织匀浆或磨浆，以提取组织中有效成分的一种提取方法，该方法具有提取完全、效率高、能耗低等特点，目前已在样品前处理中得到广泛应用 ［8