椭圆叶花锚化学成分研究.docVIP

椭圆叶花锚化学成分研究 　　摘要: 目的：对龙胆科花锚属植物椭圆叶花锚(?Halenia elliptica? D.Don) 的干燥全草进行化学成分研究.方法：椭圆叶花锚的干燥全草用90% 乙醇提取，依次用石油醚、乙酸乙酯、正丁醇萃取，对乙酸乙酯萃取部分采用各种柱色谱进行分离纯化，通过波谱数据分析 (?1H NMR，??13?C NMR，MS) 进行结构鉴定.结果：从乙酸乙酯萃取部分分离鉴定了5个化合物，分别为vogeloside (1)，?3-?表蒲公英赛醇(2)，swertanone (3)，木犀草素(4)，邻苯二甲酸丁酯异丁酯(5).结论: 化合物1~5为首次从椭圆叶花锚中分离得到. 　　? 　　关键词: 椭圆叶花锚；三萜；裂环烯醚萜苷? 　　中图分类号:R 284.1 文献标志码:A 文章编号:1672-8513(2011)05-0348-02 　　?? 　　Chemical Constituents of ?Halenia elliptica? 　　 　　WANG Hong?ling??1,2?, CHEN Hao??1,2?, GENG Chang?an?1, ZHANG Xue?mei?1, CHEN Ji?jun?1 　　? 　　 (1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese? Academy of Sciences,Kunming 650204, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China) 　　 　　Abstract: This research studies the chemical constituents of ?Halenia elliptica.? The air-dried whole plants of ?Halenia elliptica? was extracted with 90% EtOH. The EtOH extract was condensed to a small amount of volume and extracted with petroleum ether, EtOAc and ?n?-BuOH, successively. The compounds 1―5 were isolated and purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses(?1H NMR,???13?C? NMR,MS). The result showed that five compounds were isolated from ?Halenia elliptica?, and characterized as vogeloside (1), epitaraxerol (2), swertanone (3), luteolin (4), and butylisobutylphthalate (5) and compounds 1―5 were obtained from ?Halenia elliptica? for the first time. 　　? 　　Key words: ?Halenia elliptica?;triterpenoids;secoiridoid glycosides 　　 　　椭圆叶花锚(?Halenia elliptica? D.Don) 为龙胆科 (Gentianaceae) 花锚属植物，一年生草本，是“藏茵陈”类药用植物中的一种，云南民间作为青叶胆习用品种［1］，主要分布于我国西南、华北、西北各省区.全草可入药，味苦，性寒，具有清热利湿、平肝利胆之功效，用于治疗急性黄疸型肝炎、肝囊炎、胃炎、牙痛等［2］.文献报道该植物中主要??有?口山?酮及其苷、黄酮、裂环烯醚萜苷、倍半萜及三萜类化合物［3］.? 　　1 材料与仪器? 　　MS用VGAutopec-3000 型质谱仪测定；?1H NMR、??13?C NMR(DEPT)用Bruker AM-400型和DRX-500型超导核磁共振波谱仪测定，TMS为内标；柱色谱硅胶 (200～300目) 和薄层色谱硅胶板GF??254?均为青岛美高集团有限公司生产；Rp-18 薄层板和柱色谱购自Merck公司；Sephadex LH-20