骨髓间充质干细胞在糖尿病模型创面中向表皮细胞分化初步研究.docVIP

骨髓间充质干细胞在糖尿病模型创面中向表皮细胞分化初步研究 　　[摘要]目的:探讨糖尿病皮肤创面微环境中的骨髓间充质干细胞分化为表皮细胞的可能性。方法:从大鼠骨髓中分离纯化骨髓间充质干细胞。选取第3～4代细胞,应用5-溴脱氧尿嘧啶(5-BrdU)标记技术进行细胞标记;同时采用一次性腹腔内注射链脲佐菌素制作糖尿病大鼠模型。2周后,在大鼠背部人工造成圆形创面,将已标记的骨髓间充质干细胞以注射方式回植入糖尿病大鼠创面组织周围,分别于注射后2、3周取材,常规石蜡包埋、连续切片,行BrdU和角蛋白免疫组织化学染色。结果:BrdU阳性细胞出现在表皮、真皮及皮下组织各层,连续切片表皮层中部分阳性细胞同时也表达角蛋白。结论:在糖尿病溃疡愈合过程中,骨髓间充质干细胞具有向表皮细胞分化的潜能。 　　[关键词]骨髓间充质干细胞;糖尿病;创面愈合;表皮细胞;细胞分化 　　[中图分类号]Q813.11Q254[文献标识码]A[文章编号]1008-6455(2010)01-0065-03 　　 　　Preliminary study on differentiation of bone marrow mesenchymal stem cells into epidermal cells in diabetic wound 　　ZHONG Xiao-hong,WANG Ming-gang,ZHAO Li-ping,GAO Xin-yu 　　(Department of Plastic Surgery,the Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001,Anhui,China) 　　 　　Abstract:ObjectiveTo explore the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into epidermal cells under the microenvironment of diabetic wound.Methods MSCs were isolated from the myeloid tissue of the rats, purified and cultured. Third-or fourth-generation MSCs were selected and labelled with 5-bromodeoxyuridine (5-BrdU). The rats were injected intraperitoneally with streptozocin(STZ) once to establish diabetes rat model. After 2 weeks, a round skin was excised on all dorsal back of rats. BrdU-labelled MSCs were, by means of injection, engrafted to skin of the rats. The specimens were harvested from the wound tissues for paraffin embedding and serial section at the 2nd and 3rd week after transplantation and were immunohistochemically stained by either BrdU or keratin. Results BrdU positive cells aggregated in the epidermis, dermis and hypodermia. In serial section, some positive cells expressed keratin simultaneously in the epidermis. Conclusion During the course of the diabetic wound healing, MSCs have the potential for differentiating into epidermal cells. 　　Key words: marrow mesenchymal stem cells; diabetes mellitus; wound repair; epidermal cells; cell differentiation 　　 　　创面愈合是一个由细胞、细胞外基质及细胞因子