孟鲁司特对百草枯所致大鼠肺损伤治疗作用.docVIP

孟鲁司特对百草枯所致大鼠肺损伤治疗作用 　　【摘要】目的 探讨孟鲁司特对百草枯（PQ）中毒所致大鼠肺组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、肺湿/干质量比（W/D）和血清中肿瘤坏死因子（TNF-α）、白细胞介素-10（IL-10）、肺组织中核因子κB（NF-κBp65）及肺组织病理变化的影响。方法 取SD大鼠104只，随机（随机数字法）分为3组，PQ组40只、孟鲁司特组40只、对照组24只。PQ组和孟鲁司特组用百草枯一次性染毒，分别在不同处理后第1、3、5、7天时处死大鼠。观察肺组织中MDA和SOD含量、肺湿/干质量比（W/D）、血清TNF-α、IL-10含量及肺组织中NF-κBp65含量，并取肺组织制备标本分别在光镜和电镜下观察肺组织的病理改变。结果 PQ组第7天时肺组织中MDA含量、肺组织湿/干质量比、血清TNF-α和IL-10含量、肺组织中NF-κBp65含量明显高于对照组，SOD含量明显低于对照组，差异均具有统计学意义（P 　　【关键词】孟鲁司特；百草枯；中毒；肺损伤；丙二醛；超氧化物歧化酶；白细胞介素-10；核因子κB 　　Empirical study of therapeutic effect of montelukast on acute lung injury induced by paraquat in rats SUN Jian-bo*，GU Peng-yi， LI Gang， QI Qi-ge， JIN Shuai， JIA Dong， SANG Zhen-zhen， ZHAO Min.*Department of Emergency， Shengjing Hospital of China Medical University， Shenyang 110004， China 　　Corresponding author：ZHAO Min，Email：zhaom@sj-hospital.org 　　【Abstract】Objective To discuss the effect of Montelukast （Mont） on MDA，SOD，W/D，TNF-α，IL-10 and NF-κBp65 in lung tissue of Wistar rats poisoned by paraquat（PQ） and also to observe the pathological changes of the lung tissue. Methods A total of 104 Wistar rats were divided into 3 groups in random （random number）， namely PQ group （n=40）， Mont group （n=40） and control group （n=24）. PQ （20 mg/kg） was administered by intra-peritoneal route to rats of PQ group and Mont group and narcotics were used for 2 hours. Mont in dose of 50 mg/kg was administered intra-gastrically to rats of Mont group per day and saline instead were administered to PQ group and control group per day until they were sacrificed for experiment. Of both PQ group and Mont group， 10 rats were sacrificed at each interval of 1，3，5 and 7 days respectively after modeling， whereas 6 rats of control group were sacrificed at each interval. The levels of MDA and SOD in lung tissue and W/D of lung tissue， the levels of serum TNF-α and IL-10 and the level of NF-κBp65 in lung tissue were determined. Further， the specimen of lung tissue was prepared for electron microscopy observation.Results The leve