精选钩吻b组分对的hela细胞增殖和细胞周期的影响.doc

钩吻B2组分对Hela细胞增殖和细胞周期的影响 B2???a??Hela?????????????????5?????? 3 1,?lt;???? 1 ,?????p 2 (1.?C???x?????????5????,????????410078;2.?????,?H?x???U????,????????410006) g?g?[????]g?????:???????U????2(GW2B2)???a??Hela?????5???8???????????????5??????g2????:???EXTT?? ?a???????5???8????,Timelapse?w?????????????5???????B,?k?;?????????Q?????????????5,???,????GW2B2???a??Hela ?????????Eg2????:GW2B2???(?8?w???FHela?????5???8?????????E,1H?L/mLGW2B2???a?e?a??Hela?????5???5???8?w ????,?E2??5H?L/mL???J???H????,?????5???8???????????o?????????Fg2GW2B2???a?e?a?8,Hela?????U???????B?????W ?S?y???????x?P,???????????m,?????a???U??,???a?????f???y??g2GW2B2???a???8?w??????Hela????????,1H?L/mLgB 2H?L/mL??5H?L/mLGW2B2???a?e?a72h?8,Hela?????????????a?t????8116%gB36183??61132%,?0?????????5???????U ???uG2/M?5?U??,?0????G0/G1?5???(???????U?????Eg2????:GW2B2???a???(???FHela????????gB????????,???(?8?w ???????R?????????E;GW2B2???a?????????5???????U???uG2/M?5?U??,???8????????????????g2 [???n??]g????U;Hela????;????????;???????5 [?C???a????R28515]g?g?g?[???n?1???B]Ag?g?g?[?????W??]1673-016x(2008)04-0020-04 EffectofB2ComponentofGelsemiumElegansBenth onHelaCellsProliferationandCellCycle ANFei2yun 1 ,CHENCui2mei 1 ,LIANGWei2jun 2 (1.PublicHealthSchoolofCentreSouthUniversity,Changsha410078,China; 2.MedicalCollege,HunanNormalUniversity,Changsha410006,China) [Abstract]Objectiveg?TostudyoneffectofcompoundofethanolandchloroformextractionfromGelsemium elegansBenth(GW2B2component)onHelacellsproliferationandcellcycle.Methodsg?Cellproliferationwasde2 tectedbyXTTmethod,cellgrowthmorphologicalchangeswasobservedwithTime2lapseinversionmicroscope,ap2 optosisandcellcyclewasanalyzedbyflowcytometer,comprehensiveevaluatedtheeffectsofextractofGelsemiu2 moncellgrowthandproliferation.Resultsg?GW2B2componentobviouslyinhibitedHelacellgrowthandprolifera2 tion,growthspeedofHelacellwasdecreasedobviouslyatconcentration1H?L/mL,andcellgrowthandproliferation abilitywasinhibitedalmostcompletelyatconcentration2H?L/mor5H?L/mL.Distinctmorpholo