肠出血性大肠杆菌eheco157h7的分子进化分析-molecular evolution analysis of ehec eh eco 157 h 7.docxVIP

下载本文档

7
0
约9.15万字
约 100页
2018-06-28 发布于上海
举报
版权申诉

肠出血性大肠杆菌eheco157h7的分子进化分析-molecular evolution analysis of ehec eh eco 157 h 7.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

肠出血性大肠杆菌eheco157h7的分子进化分析-molecular evolution analysis of ehec eh eco 157 h 7

第三军医大学博士学位论文第三军医大学博士学位论文 PAGE PAGE 4morphology, Biochemical identification, and 16s rRNA sequence. After estabalished the strains database, the virulent factors, such as stx1/2 、 hlyA and tccP were detected.Combined with Multilocus sequence typing (MLST) for investigating populations and population dynamics on a global scale using neutral or slowly accumulating geneticvariation in housekeeping genes （aspc， icdA， uidA，clpX，mdh， fadD ，lysp ）. Toassess the genetic diversity and variability of EHEC O157:H7, and to to determine the molecular relatedness among these strains, which may provide insights into the microevolutionary history of genome divergence and to help understand sources and routes of transmission of this infection.Results:EHEC O157:H7 isolates were obtained from strain databases built by JS Province Center for Disease prevention and Control during the year 1999 to 2007, as well as from CQ municipality during 2005 to the 2007, China. MLST analysis and determination of diversity in the virluence agent :seven fragments of the housekeeping genes’ allelic loci of E. coli O157:H7 were selected for the MLST scheme. There are aspc、icdA、 uidA、 clpX、 mdh、fadD and lysp.There’re 7 STs and most had not been described previously at the EcMLST database, that were ST836、ST837、ST838、ST839、ST840、ST841 and ST842. BURST (Based Upon Related Sequence Types) algorithm showed that these 7 STs could be clustered in 2 groupsThe presence of stx1, stx2, hlyA and TccP repeat-fragments was were identified by PCR. Only 1 (99G144) isolates carried stx1 genes, whereas all possessed stx2 genes. All strains possessed the large 60-MDa pO157 virulence plasmid and were PCR positive for the enterohemolysin gene (hlyA) (100%). The sequence results of the 527bp hlyA gene are all the same. PCR results detecter 3 kinds of tccp gene repeat-types. Among study strains there are that 13 (12.4%) isolates contained 3 repeats-fragments, 6 (5.7%) contained 4 repeats, and 86 (82.0% )