不同品种怀地黄以糖类成分为指标的质量控制评价标准分析-analysis of quality control evaluation criteria for different varieties of rehmannia glutinosa libosch with sugar components as indicators.docxVIP

目录摘要...............................................................................................................................1Abstract3前言...............................................................................................................................5第一部分紫外分光光度计法测定怀地黄中糖类成分的含量.....................................6第一节紫外分光光度计法测定怀地黄中多糖含量.....................................................61实验材料....................................................................................................................62糖含量测定方法的建立............................................................................................7第二节紫外分光光度计法测定怀地黄中小分子总糖的含量...................................131实验材料..................................................................................................................132样品小分子总糖的制备..........................................................................................133样品小分子总糖的含量测定..................................................................................134结论与讨论..............................................................................................................15第二部分RP-HPLC-RID法同时测定怀地黄中单糖、低聚糖含量161实验材料..................................................................................................................162方法与结果...............................................................................................................173结论与讨论..............................................................................................................37第三部分怀地黄中低聚糖部位HPLC指纹图谱的建立39第一节鲜地黄中低聚糖部位HPLC指纹图谱的建立391仪器与试药..............................................................................................................392实验方法与结果......................................................................................................40第二节生地黄中低聚糖部位HPLC指纹图谱的建立...............................................541仪器与试药...............................................................................................................54