白藜芦醇对复杂性区域疼痛综合征1型大鼠骨折模型疗效及作用机制-effect and mechanism of resveratrol on complex regional pain syndrome type 1 rat fracture model.docxVIP

ABSTRACT Objective: To explore the action mechanism of MIF in complex regional pain syndrome type 1 and the possible mechanism of resveratrol in treatment of CRPS1, and compare the therapeutic efficacy in different doses of resveratrol to CRPS1. Methods: Part I：50 adult male SD rats were randomly divided into 7 groups: normal control group(n=5), sham-operated group(n=5), block-treated group-1(n=8), block-treated group-2(n=8), block-treated group-3(n=8), resveratrol-treated group (n=8), model control group(n=8). Block-treated group-1,2,3 were respectively given anti-CD74 antibodies[1mg/(kg·d)], PD98059[10mg/(kg·d)] and SB203580[10ug/ (kg·d)]. Resveratrol-treated group was given resveratrol[20mg/(kg·d)]. Normal control group, sham-operated group and model control group were given 5% DMSO. All of the groups were continuously intraperitoneal injected for 14 days. Compared the pain behavior in rats, the MIF and TNF-α levels in serum before and after treatment (7days, 14days). The expressions of p38MAPK and p-p38MAPK in sciatic nerve of rats were detected by western blot after treatment. Part II：50 SD rats were randomly divided into 7 groups: normal control group (n=5), sham-operated group(n=5), targeted therapy group-1(n=8), targeted therapy group-2(n=8), resveratrol-treated group-1(n=8), resveratrol-treated group-2(n=8), model control group(n=8). Targeted therapy group-1,2 were respectively given ISO-1 （ MIF inhibitor ） in doses of 0.1mg/(kg·d) and 1mg/(kg·d). Resveratrol-treated group-1,2 were respectively given resveratrol in doses of 20mg/(kg·d) and 40mg/(kg·d). Normal control group, sham-operated group and model control group were given 5% DMSO. All of the groups were continuously intraperitoneal injected for 14 days. Compared the pain behavior in rats, the MIF and TNF-α levels in serum before and after treatment (7days, 14days). The expressions of MIF in sciatic nerve of rats were detected by western blot after treatment. Results: Part I ：