共振光散射技术测定药物及生物分子新方法的研究与应用-research and application of a new method for determination of drugs and biomolecules by resonance light scattering technique.docx

英文摘要英文摘要 PAGE PAGE IVAbstractPasternack and his coworkers first established the Resonance Light Scattering (RLS) technique with a common spectrofluorometer in 1993. The RLS technique has attracted more and more interests because of its advantages including high sensitivity, good selectivity and simplicity. As a new analytical method, RLS has shown great potential significance on application and the latest investigation even has applied RLS in many new areas such as supermolecule chemistry, physical chemistry, life sciences and so on. To further study and develop high sensitivity, good selectivity, simple and convenient new RLS systems and methods has already become one of the important study subjects.In this dissertation, with muti-spectral techniques, we have established 8 new RLS methods for the determination of medicament and biomoleculars. Two parts are included in this contribution: RLS study and application on medicament and biomoleculars.Part One The determination of the medicament and the trace element contained in the medicineIn this study, a new rutin assay had been first established with Mn (II) as the RLS probe, which based on the unusual RLS decrease phenomenon. In addition, another rutin assay was established making use of the aminopyrine- cetyltrimethylammonium bromide-rutin system and based on the distinct RLS enhancement. Both the the effect of the factors and the optimum reaction conditions had been studied. In pH 7.5, the RLS decrease phenomenon was observed because of the interaction of 0.2 mg/mL Mn (II) with rutin. A good linear relationship betweenthe changes of RLS intensities of manganese sulfate with and without rutin and the concentrations of rutin was obtained over the range of 0.49 μg/mL to 24.4 μg/mL and a low detection limit (LOD, 3σ) 0.42 μg/mL was achieved in the mean time. In the RLS system of aminopyrine- cetyltrimethylammonium bromide-rutin, the surfactant was acted as a bridge. The LOD was 0.12 μg/mL and the linear range