重氮偶合分光光度法测定水中苯胺研究.docVIP

重氮偶合分光光度法测定水中苯胺研究 　　摘要：[目的] 建立水中苯胺浓度的6种重氮偶合分光光度测定法。[方法] 向含有苯胺的水样中分别加入N-（1-萘基）-乙二胺盐酸盐、α-萘胺、α-萘酚、邻甲氧基酚、8-羟基喹啉和变色酸，在亚硝酸钠作用下发生重氮偶合反应，采用分光光度法分别在545、540、495、450、480和510nm处进行测定。[结果] 6种显色体系在线性范围分别为0~5.5、0~6.0、0~6.5、0~11.5、0~10.0和0~9.5mg/L，所得苯胺的回归方程呈现较好的线性关系，检出限分别为0.009、0.011、0.014、0.032、0.025和0.022mg/L；平均回收率在95.1~105.3%范围内；RSD在1.96~4.80%范围内。采用该6种方法与气相色谱法测定水中苯胺浓度，差异均无统计学意义。[结论] 6种方法均简单、快捷、准确度高，可用于水中苯胺浓度的快速测定。 　　 　　关键词：苯胺 重氮偶合 分光光度法 亚硝酸钠 　　 　　Spectrophotometric Methods Based on Diazotion-coupling Reaction for the Determination of Aniline Concentration in Water 　　ZHAO Mu-qiu et al (College of Biological Science and Technology, Qiongzhou University, Hainan, Sanya 572022) 　　Abstract：[Objective] The aim was to establish six spectrophotometric methods for determining aniline concentration in water based on diazotion-coupling reaction. [Method] Aniline coupled with N-(1-naphthyl)-ethylenediamine, α-naphthyl amine, α-naphthol, guaiacol, 8-hydroxy- quinoline or chromotropic acid in sodium nitrite medium to form colored dye. [Result] The maximum absorption wavelength of resultant was 545、540、495、450、480 or 510nm, the linear range was 0-5.5, 0-6.0, 0-6.5, 0-11.5, 0-10.0 or 0-9.5mg/L, and the limit of detection was 0.009, 0.011, 0.014, 0.032, 0.025 or 0.022 mg/L, respectively. The rates of recovery were 95.1-105.3%, and RSDs were 1.96-4.80% respectively. Compared with gas chromatography, no significant difference presented using these 6 methods determining aniline concentration in waste water. [Conclusion] These six methods are simple and accurate and applicable to the determination of aniline concentration in water. 　　 　　Key words Aniline：Diazotion-coupling Spectrophotometric Method Sodium Nitrite 　　 　　苯胺作为重要的有机化工原料和精细化工中间体广泛应用于制药、印染和纺织等行业，是一种具有芳香气味的无色油状液体 [1]。随着工业的发展，越来越多的苯胺随工业废气和污水进入环境水体和农业水体。因其毒性较大，对水体等生态环境和人类健康存在潜在威胁，已被列入我国环境优先污染物名单[2]。苯胺是典型高铁血红蛋白的形成体，能使细胞失去携氧功能，造成溶血性贫血，损害肝脏引起中毒性肝炎，甚至导致各种癌症[3]。正因为苯胺对人体的危害极大，所以环境中尤其是农业用水中苯胺的残余量引起人们的极大关注，水环境中的苯胺测定已被列入环境监测的经常性项目[4]。 　　水体中苯胺浓度的测定方法有光度