探究无花果含药血清对C6胶质瘤细胞抑制作用及凋亡的影响.pdfVIP

提 要 目的：研究中药无花果对 C6 胶质瘤细胞生长的抑制作用，并探讨其作用机制。 方法：采用体外细胞培养及肿瘤细胞大鼠体内种植的方法研究无花果含药血清对 C6 胶质瘤细胞生长的抑制作用。体外实验中，运用不同浓度含药血清干预细胞，观察其 对细胞增殖和细胞调亡的影响；体内实验中，建立大鼠颅内脑胶质瘤模型，观察病理 形态和组织学形态。结果：体外实验中，显示了对 C6 胶质瘤细胞不同程度的抑制作 用，以中等浓度含药血清效果最好，并可以增强化疗药物的疗效。体内实验，大鼠 C6胶质瘤模型建立成功率100%。结论：无花果含药血清对C6胶质瘤有十分确切的治 疗作用，并可以抑制胶质瘤细胞的生长，促进 C6 胶质瘤细胞的凋亡，其研究结果为 脑胶质瘤的中西医结合临床治疗提供了新的思路和方法。 关键词 无花果；C6 胶质瘤；抑制作用；细胞凋亡；细胞增殖；大鼠颅内胶质 瘤模型；实验研究 Experimental Study on the Inhibition Effect of Ficus carica upon Glioma on Rats Speciality : Clinical study of Neurosurgery of Integrated of Traditional Chinese Medicine and Modern Medicine Author : Tao Han Tutor : Prof. Ruming Jiang ABSTRACT Objective: The study is focus on the inhibition effect of natural plant Ficua carica L. (fig) on C6 glioma, and explores the mechanisms of the effect in an experimental research. Methods: The inhibition effects of fig and its various constituents and preparations were studied in vitro and on a C6 glioma-bearing rats model in vivo. Concentrations of fig preparations were used and studied their effects on cell proliferation in vitro. The intracranial C6 glioma-bearing rats model was established, to observe pathomorph and histology form. Results: Inhibition effects on glioma cell were showed in every concentration group of various preparations in vitro, the fig latex and middling concentration groups showed most significant effect. in vivo, Achievement ratio of glioma model of rat Intracal is 100%. Conclusion: Blood serum of containing fig drug showed an affirmative curing effect on glioma both in vitro, and can refrain the growth of glioma cell a