慢性乙型肝炎患者cd4cd25treg及部分细胞因子变化的研究-changes of cd 4 cd 25 treg and some cytokines in patients with chronic hepatitis b.docxVIP

汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 PAGE PAGE IV is no correlation between CD4+CD25+Treg and ALT both in the groups of CHB and AHB(r=0.196, P0.05; r=-0.368, P0.05); 2 The levels of IL-2, IL-4, IL-10, IFN-γ and TGF-β in the group of CHB are 31.27 ±3.16pg/ml, 52.12±2.65pg/ml, 165.78±11.33 pg/ml, 76.25±1.70 pg/ml and 10.96 ±0.83 pg/ml respectively, while in the group of normal control are 14.68±1.77 pg/ml, 25.06±2.66 pg/ml, 84.49±3.21 pg/ml, 43.00±2.92 pg/ml and 5.73±0.64 pg/ml respectively. The level of each cytokine above the fine ones in the group of CHB is higher than that of the group of normal control (P0.01). There is a positive correlation between ALT and IL-2、IFN-γ respectively in the group of CHB(r=0.442,P0.05;r=0.469,P0.05). Conclusion There exists immunologic derangement in patients with chronic hepatitis B; CD4+CD25+Treg in peripheral blood and Th1/Th2 imbalance play an active role in the chronicity of hepatitis B. Key Words : chronic hepatitis B；CD4+CD25+Treg；Th1/Th2 imbalance；cytokines 汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 英文缩略词 缩略词 英文全称 中文全称 HBV hepatitis B virus 乙型肝炎病毒 Treg T regulatory cell 调节性 T 细胞 CHB chronic hepatitis B 慢性乙型肝炎 AHB acute hepatitis B 急性乙型肝炎 IFN-γ interferon-γ 干扰素-γ TGF-β transforming growth factor-β 生长转化因子-β IL-2 interleukin-2 白细胞介素 2 IL-4 interleukin-4 白细胞介素 4 IL-10 interleukin-10 白细胞介素 10 ALT alanine aminotransferase 谷丙转氨酶 HBsAg hepatitis B surface antigen 乙型肝炎表面抗原 HBsAb hepatitis B surface antibody 乙型肝炎表面抗体 HBeAg hepatitis B e antigen 乙型肝炎 e 抗原 HBeAb hepatitis B e antibody 乙型肝炎 e 抗体 HBcAb hepatitis B core antibody 乙型肝炎核心抗体 RT reverse transcription 逆转录 PCR polymerase chain reaction 聚合酶链式反应 ELISA enzyme linked immunosorbent assay 酶联免疫吸附法 DNA deoxyribonucleic acid 脱氧核糖核酸 Foxp3 transcription factor forkhead box P3 叉头翼螺旋转录因子 FITC fluorescein isothiocyanate 异硫氰酸荧光素 PE phycoerythrin 藻红素 PBS phosphate buffered solution