铅对发育期大鼠海马细胞凋亡的诱导和xiap smac基因表达的影响-effects of lead on induction of apoptosis and expression of xiap smac gene in developing rat hippocampal cells.docxVIP

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2018-07-28 发布于上海
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铅对发育期大鼠海马细胞凋亡的诱导和xiap smac基因表达的影响-effects of lead on induction of apoptosis and expression of xiap smac gene in developing rat hippocampal cells.docx

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铅对发育期大鼠海马细胞凋亡的诱导和xiap smac基因表达的影响-effects of lead on induction of apoptosis and expression of xiap smac gene in developing rat hippocampal cells

AbstractBackground:Leadisaubiquitouspollutantintheenvironment.Brain,especiallythenervoussystemindevelopmentphase,ismorevulnerabletodeleteriouseffectsofleadthanothertissues.Theapoptosishavebeensuggestedtobeacausativefactorinleadneurotoxicity,butlittleinformationonthisprocesshasappearedtodate.Theapoptosisprocessisregulatedbymanyfactors.ExperimentalstudieshaveshownthegeneXIAP(XChromosomeLinkedInhibitorofApoptosisProtein)isanimportantfactorintheprocessofrepressingtheapoptosis,whichisthestrongestendogenousinhibitorofthecapaseuptonow.ThechondrosomebindingproteinSmac(Smac/DIABLO，secondmitochondria-derivedactivatorofcaspase/directinhibitor-of-apoptosisprotein-bindingproteinwithlowpI)caninhibittheXIAPrepressingthecaspase,activatethecaspaseenzymeprecursor,andenhancetheenzymeactivityofthecaspase,whichhasreceivedmanyresearchers’considerableattentionrecently.Objective:ToprovidesomescientificbasisfortherevealmentofLeadneurotoxinmechanism,thepresentstudywasundertakentoobservetheeffectofleadacetateontheapoptosisandtheexpressionofapoptosis-relatedgeneXIAPandSmacinhippocampusofbuddingrates.Method:48healthymaleSprague-Dawley30-day-oldratswererandomlydividedinto4groupswith12ratsineachgroup:distilledwaternegativecontrolgroup,2mg/Kg、20mg/Kg、200mg/Kgleadexposuregroup.Leadacetateorwaterwasgiventhroughgastricperfusionfor6weeksrespectively.The12ratsofeachgroupwererandomlydividedintotwosubsets,with6ratsineachsubset.Theratsinthefirstsubsetwereexecutedbydecapitation,andthenthebloodwascollectedtomeasuretheleadconcentrationinbloodbyatomicabsorptionspectrophoto-metry.Thehippocampustissuewasseparatedfromthebrainoftheratsinthefirstsubset.TheexpressionofXIAPandSmacgenesinhippocampuswasobservedbyRT-PCRmethod.Therightbrainsoftheratsinthesecondsubsetwerefixed,dehydrated,paraffinimbeddedandwasmadeintoparaffinsections.ThedeterminationofapoptosisinhippocampuswasmadebyTUNEL(terminal-deoxynucleotidyltransferasemediated-UTPnickandlabeling)stain.Result:TUNELstainshowedthatthereweresignificantdifferencesbetween