铅诱导大鼠脑组织氧化应激损伤及对其xrcc1和parp1基因mrna表达的影响-oxidative stress injury induced by lead in rat brain and its effect on mrna expression of xr cc1 and par p1 genes.docxVIP

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2018-07-28 发布于上海
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铅诱导大鼠脑组织氧化应激损伤及对其xrcc1和parp1基因mrna表达的影响-oxidative stress injury induced by lead in rat brain and its effect on mrna expression of xr cc1 and par p1 genes.docx

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铅诱导大鼠脑组织氧化应激损伤及对其xrcc1和parp1基因mrna表达的影响-oxidative stress injury induced by lead in rat brain and its effect on mrna expression of xr cc1 and par p1 genes

ABSTRACTAsatoxicheavymetal,leadexistsintheenvironmentwidelyandcancausedamagesofmultiplesystemsandorgans.Nervoussystemissensitivetolead,andevenalittleleadexposurecancausethedysfunctionofthenervoussystem.Oxidativestressdamageisconsideredasoneofthepossiblemechanismsofnervoussystemdamageinducedbylead.Theoverproductionofreactiveoxygeninducedbysomedamagefactorscandestroythebalancebetweenoxidationandanti-oxidationsystemsoastoleadtoastateofoxidativestressandresultantdamagestoDNA,proteinandlipids.IntherepairprocessofDNAoxidativedamage,twoofthemostimportantsignalingmoleculesareXRCC1andPARP1.Objective:ToobservetheeffectsofleadexposurethroughdrinkingwaterontheoxidativestressindicatorsandtheexpressionofXRCC1andPARP1mRNAincerebralcortex,cerebellumandhippocampusofratssoastoprovidenewcluesandbasisforthefurtherstudyonleadneurotoxicitymechanism.Methods:40weanedmalehealthySPFSDratsweredividedrandomlyintofivegroupsaccordingtotheweightblockdesign,anddrankunlimitedlydistilledwatercontaining0mg/L,100mg/L,200mg/L,400mg/L,800mg/Lleadacetatefor60daysrespectively.Thenthelevelsofleadinblood,cortex,cerebellumandhippocampusweredetectedbygraphitefurnaceatomicabsorptionspectrometry.TheactivityofSODandthecontentsofGSH,H2O2andMDAinbraintissueweredeterminedwiththerelatedkitsrespectively.TheexpressionofXRCC1andPARP1mRNAinthebrainwasdetectedwithPT-PCRtechnique.Results:(1)Theconcentrationsofleadintheblood,cerebralcortex,cerebellumandhippocampusofleadexposuregroupswerehigherthanthatofcontrolgroup(P＜0.05).(2)Comparedwithcontrolgroup,SODactivityandGSHcontentincerebralcortex,cerebellum,hippocampusoflead-exposedratsdecreased(P0.05),whereascontentsofH2O2andMDAincreasedsignificantly(P0.05).CorrelationanalysisresultsrevealedthatSODactivityandGSHcontentincerebralcortex,cerebellumandhippocampusofratswerenegativelycorrelatedwithbloodleadlevels(thecorrelationcoefficientwere-0.477,﹣0.391,﹣0.383,﹣0.530,﹣0.558,﹣0.420respectively,P＜0.05),whileH2O2andMDAcontentswerepositivelycorrelatedwithbloodleadlevels(thecorrelationc