水平潜流人工湿地氮循环微生物效应及生态模型分析-microbial effects and ecological model analysis of nitrogen cycle in horizontal subsurface flow constructed wetland.docx

ThemicrobialeffectsonnitrogencyclingandecologicaldynamicsmodelinhorizontalsubsurfaceflowconstructedwetlandsAbstractTheperformanceandmechanismsofhorizontalsubsurfaceflowcontructedwetlandsreed/gravelbedsystemofGuantingReservoirwerestudiedforthepreventivetreatmentofthepollutionofpotablewatersources.Thestudyincludes:(1)TheNitrogenremovalrateandmainfactoranalysisofhorizontalsubsurfaceflowconstructedwetlands;(2)Theformsdistributionandtransformationrule;(3)TheanalysisofthefrictionaldistributionandpopulationofNitrogentransformationmicrobesinthesystem;(4)Themicrobialdiversityresearchinthesystem;(5)Thefeatureddenitrifierswerescreened,thephysiologicalandbiochemicalcharacteristicswereanalyzedandidentified;(6)Modelingofnitrogencyclesinhorizontalsubsurfaceflowcontructedwetlands.Themajorresearchfindingsareasfollows:Ontheconditionthattheaveragehydraulicstayedfor3.85hours,throughNitrogenremovalanalysisofhorizontalsubsurfaceflowconstructedwetlands.Thesystemexhibitscertaindecontaminationability.TheinfluentCOD、NH4+-N、NO3--N、Org-NandTNare7.8~12.7mg·L-1、0.05~15.0mg·L-1、1.3~5.2mg·L-1、0.66~5.18mg·L-1、2.2~20.0mg·L-1.Theannualaverageremovalrateofthegravelbedbelowthereedwetlandssystemare25.8%、10.0%、42%、31.2%and18.9%.Seasonalvariationhasasignificanteffectontheremovalrateofthepollutedelements.SystemremovalratehasalittledecreaseintheelementsofCOD、NH4+-N、NO3--N、Org-NandTNincoldseason.Removalrateofeveryelementsareabout11.2%、6.5%、31.0%、20.5%and15%.Seasonalvariationhasasignificanteffectonthefactorsoftemperatureandpollutionloadrate.Iftheinfluenttemperaturechangesfrom0.9℃to24.8℃,apositivecorelationisfoundbetweentheremovalrateofvariousformsofNitrogenelements’volumeandthetemperature.TheconstantsKvofNH4+-N、NO3--N、Org-NandTNare0.01~0.2d-1、0.03~0.3d-1、0.07~0.3d-1and0.02~0.15d-1.Meanwhile,thevolumeremovalrateconstantsKv20are0.0769、0.1673、0.1508and0.0732d-1whenthetemperatureis20℃.ItinterpretsthatthewetlandshavestrongercapabilitytoreduceNO3--NandOrg-NthanNH4+-N.TheremovalrateofNH4+-NandOrg-Narelogarithmsrelat