五甲基槲皮素对糖尿病肾病大鼠的保护作用及机制分析-protective effect and mechanism analysis of pentamethyl quercetin on diabetic nephropathy rats.docx

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2018-08-07 发布于上海
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五甲基槲皮素对糖尿病肾病大鼠的保护作用及机制分析-protective effect and mechanism analysis of pentamethyl quercetin on diabetic nephropathy rats.docx

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五甲基槲皮素对糖尿病肾病大鼠的保护作用及机制分析-protective effect and mechanism analysis of pentamethyl quercetin on diabetic nephropathy rats

AbstractPentamethylquercetin(PMQ)isamethylationproductofquercetin.Ithassignificantpharmacokineticandpharmacodynamicadvantages.ItisconfirmedthatPMQhasavarietyofeffects,includinganti-inflammation,antioxidation,anti-myocardialischemiareperfusioninjury,anti-arrhythmiaactivity,anti-metabolicsyndromeeffect,etc.Ithasbeenreportedthatflavonoidcompoundshavenotableeffectsondiabetesanditsmajorcomplication,diabeticnephropathy(DN).Additionally,mesangialcells(MC)playakeyroleinDN.Inthisstudy,ratmodeloftype2diabeticnephropathywassuccessfullyestablishedtoinvestigatetherenalprotectiveeffectofPMQandthepossiblemechanism.Thecellmodelofhighglucose-inducedproliferationofMCwaspreparedtofurtherresearchtheanti-DNmechanismofPMQ.OHHOOOHOHHOOQucercetinOCH3H3COOOCH3OCH3OOCH3PentamethylquercetinPart1Establishmentofratmodeloftype2diabeticnephropathyObjective:Toestablishratmodeloftype2diabeticnephropathy.Methods:Attheageof2days,maleneonateratsweretreatedwithSTZandcontrolgroupwiththevehicle(i.p.).Bodyweightandtheconsumptionofwaterandfoodwererecordedeverydayfromtheageof6weeks.Attheageof12and16weeks,fedandfastingbloodglucose,triglyceride(TG),fastingseruminsulinlevelweretestedrespectively.Attheageof16weeks,oralglucosetolerancetest(OGTT)wasperformedandserumcreatininewastested.Attheageof16weeks,eachgroupofratswereputintometaboliccagesforthemeasurementofurinevolume,urinespecificgravity,urinaryalbuminandurinarycreatinine(Ucr),andthentheendogenouscreatinineclearancerate(Ccr)andurinaryalbuminexcretionrate(UAER)werecalculated.Afterdecapitation,bothkidneysoftheratswereisolatedandkidneyindexwascalculated.Pathologicalchangeswerealsoobserved.Results:1)TreatingneonateratwithSTZcouldsuccessfullyestablishtype2diabeticratmodel.Duringthewholegrowingperiod,themodelgroupgainedlessbodyweightbutconsumedmorewaterandfoodcomparedwiththecontrolgroup.Attheageof16weeks,increasedleveloffedbloodglucose,TGandenlargedareaundertheOGTTcurvewereobserved,whileseruminsulinleveldecreased.2)Attheageof16weeks,significantcharacteristicsofdia