通过rna-seq初步考察铜绿假单胞菌噬菌体pap3对宿主转录组的全局性调控作用-rna - seq was used to investigate the global regulatory effect of pseudomonas aeruginosa phage pap 3 on host transcriptome.docxVIP

第三军医大学硕士学位论文  PAGE 3 缩略语表 英文缩写 cDNAs英文全称 complementary DNAs中文全称 互补 DNACOGcluster of orthologous groups聚类分析ddH2O DEGsdeionic distilled water differentially expression genes去离子蒸馏水 差异表达基因dNTPdeoxyribonucleoside triphosphate脱氧核苷三磷酸EBethidium bromide溴乙锭ECFExtracytoplasmic function sigma factors细胞外功能 δ 因子EDTAethylenediamine tetraacetic acid乙二胺四乙酸FDRFalse Discovery Rate错误发现率ggram克GOGene Ontology基因本体论数据库hhour小时KEGG Kyoto Encyclopedia of Genes and Genomes 京都基因和基因组数据库 kg kilogram 千克 LB Luria-Bertani medium LB 培养基 M molar per liter 摩尔每升 min minute 分钟 ml milliliter 毫升 ncRNA non-coding RNA 非编码 RNA nt nucleotide 核苷酸 OD optical density 吸光度 PA3Pseudomonas Aeruginosa-3铜绿假单胞菌3分离株PAGEpolyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳PaP3Pseudomonas Aeruginosa phage-3铜绿假单胞菌噬菌体3分离株PCRpolymerase chain reaction聚合酶链式反应RINRNA Integrity NumberRNA 完整性值 RNAPRNA polymeraseRNA 聚合酶SDShine-Dalgarno sequence核糖体结合序列sRNAsmall non-coding RNA小非编码 RNATUsTranscriptional Units转录本UTRsUntranslated Regions非翻译区VFBDThe Virulence factor database毒力因子数据库μlmicroliter微升 Application of RNA-seq to Investigate the Global Transcription Regulation of Pseudomonas Aeruginosa phage PaP3 on Its Host Bcaterium Abstract P.aeruginosa is a gram-negative human pathogen of Pseudomonadaceae existing in soil and water. It is a typical opportunistic pathogen can cause infections in pulmonary tract,urinary tract,burns and wounds.Treatment of Pseudomonas aeruginosa infection has been difficult because it can develop multi-drug resistance to many antibiotics. The resistance to antibiotics is inherently resistant but can also acquire resistance genes from other bacteria. For several years, numerous studies have focused on elucidating the mechanisms of pathogenesis and antibiotic resistance of P. aeruginosa. Since the sequencing of its genome, the emphasis of these studies has shifted from conventional pathogenesis research, where the focus was o