Ang2VEGF及MVD在胃癌中表达及其临床意义.docVIP

Ang2VEGF及MVD在胃癌中表达及其临床意义 　　[摘要] 目的 探讨血管生成素-2(Ang-2)、血管内皮生长因子(VEGF )在胃癌组织中表达及其与微血管密度(MVD)和临床病理特征的关系。方法应用免疫组化两步法检测51例胃癌组织中Ang-2、VEGF 和CD34 的表达，并以24例正常胃黏膜标本作对照。对CD34 阳性血管进行MVD计数，并结合临床资料进行统计学分析。结果 胃癌组织中的Ang-2及VEGF阳性表达率分别为60.8%、53%，MVD为31.9±11.2；正常胃黏膜组织Ang-2及VEGF阳性表达率分别为12.5%、4.17%，MVD为13.1±4.3，胃癌组织中Ang-2、VEGF表达率及MVD均明显高于正常胃黏膜组织(均?PAng-2表达与胃癌的大小、有无淋巴结转移、浸润深度、TNM分期有关（均P＜0.01），而与患者年龄、性别、胃癌的分化程度无关。在胃癌组织中Ang-2的表达与VEGF、MVD呈正相关（分别为r=0.846，P＜0.01；r=0.71，P＜0.01）。VEGF表达与胃癌的浸润深度、分化程度、胃癌的大小、有无淋巴结转移、TNM分期有关（P0.05或P＜0.01），胃癌组织中VEGF的表达与MVD呈正相关（r=0.858，P＜0.01）。MVD与胃癌的浸润深度、有无淋巴结转移、TNM分期有关（均P＜0.01）。结论 在胃癌组织中，Ang-2、VEGF的过度表达可能在肿瘤的血管生成和进展过程中起着重要作用。? 　　[关键词] 胃癌；血管生成素-2；血管内皮生长因子；血管生成? 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1671-7562（2008）02-0086-06?? 　　 　　Expression and significance of Ang-2，VEGF and MVD in gastric carcinoma?? 　　CHENG Xu-hui，OU Xi-long,YAN Fang?2,GUAN Yun-yan?2,YANG Liu?2，SONG Yong-qian?3 　　(1.Department of Internal Medicine,Xuzhou Guandao Hospital,Xuzhou 221008,China;2.Department ?of Gestroenterology，Zhongda Hospital,Southeast University，Nanjing 210009，China;? 　　3.Department of Pathology,Xuzhou Central Hospital,Xuzhou 221000,China)?? 　　Abstract:Objective To investigate the expression of Angiopoietin-2(Ang-2)，vascular endothelial growth factor(VEGF)in gastric carcinoma and to explore their relationship with microvessel density(MVD)and clinicopathological features.Methods The expressions of Ang-2、VEGF were studied by immunohistochemistry in 51 gastric carcinoma specimens and 24 normal tissues as control.The microvessel density（MVD）was ?assayed by CD34 positive blood vessels counting.Results The positive rates of Ang-2,VEGF were 60.8%,53% respectively,and MVD was 31.9±11.2 in gastric carcinoma.However,in normal gastric tissues,they were 12.5%,4.17% and 13.1±4.3 respectively.Expressions of Ang-2,VEGF proteins were significantly higher in gastric carcinoma than those in normal tissue(all P had a positive correlation with VEGF and MVD（r=0.846，P＜0.01 and r=0.71，P＜0.01 ?respec