OK―432肿瘤疫苗对小鼠肝癌细胞Hca―F抑制作用研究.docVIP

OK―432肿瘤疫苗对小鼠肝癌细胞Hca―F抑制作用研究 　　[摘要] 目的 研究探讨OK-432肿瘤疫苗对小鼠淋巴高转移肝癌细胞Hca-F的抑制作用。 方法 对DBA/2小鼠背部接种高转移肝癌细胞Hca-F，建立肿瘤转移模型，设对照组和OK-432肿瘤疫苗组，3周后测量肿瘤大小，并绘制肿瘤生长曲线，检测各组抑瘤率、淋巴结转移抑制率，淋巴结石蜡切片HE染色观察两组淋巴细胞数。 结果 OK-432肿瘤疫苗具有明显的抑瘤作用，抑瘤率达68.4%；淋巴结转移抑制率为31.6%；通过比较对照组和给药组淋巴结的HE染色，发现给药组淋巴结内淋巴细胞明显多于对照组。 结论 OK-432肿瘤疫苗能够有效抑制肿瘤生长，促进机体免疫应答，抑制肿瘤的淋巴结转移。 　　[关键词] OK-432肿瘤疫苗；淋巴结转移；抑瘤率 　　[中图分类号] R739.86 [文献标识码] A [文章编号] 1673-9701（2015）01-0001-03 　　Research on inhibitory effect of OK-432 tumor vaccine on mice liver cancer cells Hca-F 　　QIAO Sha ZHAO Bin 　　Department of Stomatology， Shanxi Medical University， Taiyuan 030001，China 　　[Abstract] Objective To investigate the inhibitory effect of OK-432 tumor vaccine on mice highly lymph-metastatic liver cancer cells Hca-F. Methods Highly metastatic liver cancer cells Hca-F were planted in the back of DBA/2 mice and the tumor metastasis model was built. The control group and the OK-432 tumor vaccine group were set up， with 20 mice in each group. Three weeks later， the tumor size was measured， the tumor growth curve was drawn， the tumor inhibition rates and lymph node metastasis inhibition rates were detected， and the numbers of lymphocyte of the two groups were observed based on lymph gland paraffin section HE staining. Results OK-432 tumor vaccine had obvious tumor inhibition effects， with the tumor inhibition rate of 68.4%； The lymph node metastasis inhibition rate was 31.6%； Comparison of HE staining of the control group and the medication lymph node group showed that the number of lymphocyte in lymph node of the medication group was significantly larger than that of the control group. Conclusion OK-432 tumor vaccine can effectively inhibit tumor growth， promote body immune response and inhibit lymph node metastasis of tumor. 　　[Key words] OK-432 tumor vaccine； Lymph node metastasis； Tumor inhibition rate 　　近年来，肿瘤的发病率逐年增高且越来越年轻化，对患者的生命和健康造成严重的影响。目前全世界都在对肿瘤的预防和治疗进行研究。随着肿瘤药理学、分子生物学及免疫学等相关学科的快速发展和交叉渗透，肿瘤疫苗作为肿瘤治疗、放疗、化疗后的一种辅助治疗手段越来越受到人们的关注，本研究所用肿瘤疫苗