MCM2和E2F3在脑膜瘤中表达及临床意义.docVIP

MCM2和E2F3在脑膜瘤中表达及临床意义 　　[摘要] 目的 探讨脑膜瘤中MCM2、E2F3的表达与临床及病理特征的关系。 方法 收集2013～2015年西南医科大学附属医院神经外科手术切除并经病理确诊的脑膜瘤标本69例以及2015年脑外伤患者行内外减压术采集的正常脑膜19例，运用免疫组化检测MCM2、E2F3在脑膜瘤以及正常脑膜中的表达，分析瘤组织中MCM2、E2F3的表达与脑膜瘤临床及病理特征的关系。 结果 脑膜瘤中MCM2、E2F3的表达强度明显高于正常脑膜（P 0.05）。脑膜瘤中的MCM2与E2F3表达呈正相关（P 0.05）。 结论 脑膜瘤中MCM2、E2F3的表达水平显著增高，并且与病理分级程度密切相关，在调控脑膜瘤细胞的异常增殖方面起协同作用，值得临床引起重视。 　　[关键词] 微小染色体维持蛋白2；E2F3；脑膜瘤；免疫组化 　　[中图分类号] R739.45 [文献标识码] A [文章编号] 1673-7210（2016）09（c）-0039-04 　　[Abstract] Objective To discuss the relationship between the expression of MCM2 and E2F3 in meningioma， and the clinical and pathological characteristics. Methods 69 cases of meningioma specimens derived from operation resection and diagnosed by pathology in the Department of Neurosurgery， the Affiliated Hospital of Southwest Medical University from 2013 to 2015， and 19 cases of normal meningeal collected from patients with cerebral trauma by internal and external decompression in 2015 were collected. Immunohistochemistry was applied to detect the expression of MCM2 and E2F3 in meningioma and normal meningeal. The relationship between the expression of MCM2 and E2F3 in meningioma， and the clinical and pathological characteristics of meningioma were analzyed. Results The expression intensity of MCM2 and E2F3 in meningioma was significantly higher than that in the normal meningeal tissues （P 0.05）. The correlation between the expression of MCM2 and E2F3 in meningioma was positive （P 0.05）. Conclusion The expression of MCM2 and E2F3 in meningioma increased significantly and correlated with pathological grading of meningioma closely. Both of them may have synergistic effect on regulating abnormal proliferation of meningioma cells. Therefore， it is worthy of promotion in clinic. 　　[Key words] MCM2； E2F3； Meningioma； Immunohistochemistry 　　部分脑膜瘤由于生长部位特殊、毗邻周围重要组织结构、侵袭性生长或具恶性生物学特征等原因，手术不能全切肿瘤致肿瘤残留或术后复发，从而影响患者预后[1-2]，因此有必要从生物学角度寻找新的突破点探讨脑膜瘤发生及演变过程。MCM2仅表达于增殖状态细胞周期，研究表明多种肿瘤的发生与MCM2的高表达有关[3-5]。E2F3作为E2F转录因子家族成员，通过调控CyclinE、CDK2等多种